Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. subsp. tuberosum and the wild species Solanum circaeifolium Bitter

Authors

  • Rosa Espejo Universidad Nacional Agraria. Departamento de Biología
  • Giselle Cipriani New York University. Department of Biology
  • Genoveva Rosel Internacional Potato Center. Genetic Resourses Conservation and Characterization
  • Alí Golmirzaie University of Arkansas. Department of Horticulture.
  • William Roca Internacional Potato Center. Genetic Resourses Conservation and Characterization

DOI:

https://doi.org/10.15381/rpb.v15i1.1678

Keywords:

Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis.

Abstract

Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. and the diploid wild species S. circaeifolium. Fusion-treated protoplasts were cultured in V-KM medium supplemented with bovine serum albumin. First cell divisions occurred within 3—4 days. A rapid calli proliferation was observed after colonies developed. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Chromosome observations indicated that all hybrids were aneuploids. The morphology of fusion-derived regenerants was intermediate between the donor parents. This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.

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Published

04/14/2008

Issue

Vol. 15 No. 1 (2008)

Section

Articles

License

Copyright (c) 2008 Rosa Espejo, Giselle Cipriani, Genoveva Rosel, Alí Golmirzaie, William Roca

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How to Cite

Espejo, Rosa, Giselle Cipriani, Genoveva Rosel, Alí Golmirzaie, and William Roca. 2008. “Somatic Hybrids Obtained by Protoplast Fusion Between Solanum Tuberosum L. Subsp. Tuberosum and the Wild Species Solanum Circaeifolium Bitter”. Revista Peruana De Biología 15 (1): 73-78. https://doi.org/10.15381/rpb.v15i1.1678.