ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF PASTEURELLA MULTOCIDA AND GALLIBACTERIUM ANATIS STRAINS IN POULTRY WITH RESPIRATORY SIGNS

Authors

  • Gonzalo Castillo Carrera Profesional de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad Alas Peruanas, Lima, Perú
  • Ysabel Koga Bioservice SRL, Lima
  • Arnaldo Alvarado Laboratorio de Patología Clínica y Biología Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Robert Tinoco Bioservice SRL, Lima
  • Daniel Fernández Bioservice SRL, Lima

DOI:

https://doi.org/10.15381/rivep.v25i4.10812

Keywords:

broiler, hen, duck, microbial fermentation, avian respiratory syndrome

Abstract

The aim of the study was to identify biovars of Pasteurella multocida and Gallibacterium anatis in poultry with respiratory signs. These bacteria were isolated from samples of secretions and organs of affected broilers, layers and ducks from various poultry farms in the coast and the tropics of Peru. Out of 25 isolates obtained, 13 strains of P. multocida and 12 of G. anatis were identified based on culture characteristics, morphology and biochemical tests (oxidase, catalase, indole, urease). These strains were subjected to micro-well fermentation tests with 10 carbohydrates and one amino acid for typification. Results showed that eight strains of P. multocida corresponded to biovar 1 and the others to biovars 3, 4, 6, 10 and 11, while 11 strains of G. anatis corresponded to biovar haemolytica and one strain to biovar anatis.

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Author Biographies

  • Gonzalo Castillo, Carrera Profesional de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad Alas Peruanas, Lima, Perú
  • Ysabel Koga, Bioservice SRL, Lima
  • Arnaldo Alvarado, Laboratorio de Patología Clínica y Biología Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Robert Tinoco, Bioservice SRL, Lima
  • Daniel Fernández, Bioservice SRL, Lima

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Published

2014-12-30

Issue

Section

Artículos Primarios

How to Cite

Castillo, G., Koga, Y., Alvarado, A., Tinoco, R., & Fernández, D. (2014). ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF PASTEURELLA MULTOCIDA AND GALLIBACTERIUM ANATIS STRAINS IN POULTRY WITH RESPIRATORY SIGNS. Revista De Investigaciones Veterinarias Del Perú, 25(4), 516-522. https://doi.org/10.15381/rivep.v25i4.10812