Chemical evaluation and antibacterial activity in vitro of essential oil of Erythroxylum coca Lam. Var. “Coca Huánuco”: design of a pharmaceutical formulation.
Keywords:
Erythroxylum coca Lam, antioxidant, antibacterial, Streptoccocus mutans ATCC 35668, toothpaste.Abstract
Objective: To elucidate the composition chemistry of essential oil of Erythroxylum coca Lam.var. “Coca Huánuco” and determine their in vitro antioxidant and antibacterial activity against Streptoccocus mutans ATCC 35668 and the design of a pharmaceutical formulation. Design: Experimental, analytical, longitudinal and prospective study. Place: Three institutes and a research centre of San Marcos University. Biological material: coca leaves and cariogenic bacteria. Methods: preliminary analysis and structural elucidation by chromatography of Gases/spectrometry (GC/MS). In vitro antioxidant activity was carried out by DPPH, CDP and uptake of the superoxide anion radical. Antibacterial activity in vitro was made by the method of microdilusion colorimetric and diffusion in agar. Developed toothpastes containing essential oils of coca, mint and oregano to 1%. Results: The GC/MS analysis, identified the following chemicals: β-pinene, β-myrcene, stearic acid, neroridol, Phytol, acid methyl ester 7, 10, 13 hexadecatrienoico and nonacosano. The fitocompuestos of oil showed antioxidant capacity. Conclusions: The essential oil expressed antioxidant capacity that would participate in biological redox systems and a minimum inhibitory concentration (MIC) of 0,625µL/mL against Streptoccocus mutans ATCC 35668 and expressing inhibition zone of 37mm and 38mm intoothpastes.
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Copyright (c) 2016 Américo Castro, Silvia Suárez, Norma Ramos, Mario Carhuapoma, Julio Ruiz, Mario Alcarraz, Sixto Gonzales, Luis Inostroza, Omar Santa María, Ruth Lucas, Walter Vicente
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Creado a partir de la obra en http://revistasinvestigacion.unmsm.edu.pe/index.php/Theo/index.
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