Frecuencia genotípica para la presencia de TPA-25 en una población de estudiantes universitarios de Lima-Perú

Authors

  • Paola M. Casas Laboratorio de Biología Molecular, Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, Lima, Perú.
  • Amparo I. Zavaleta Laboratorio de Biología Molecular, Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, Lima, Perú.
  • Héctor C. Cataño Laboratorio de Biología Molecular, Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, Lima, Perú.

DOI:

https://doi.org/10.15381/ci.v9i2.5979

Keywords:

TPA-25, Alu insertions, SINE, population genetics

Abstract

The maín objectíve of this study was to evaluate the genotype frequency for the presence of the Alu TPA-25 insertíon in fue 8 intron of the tíssue plasminogen activator gene (TPA) in a population of 50 students belonging to the Faculty of Pharmacy and Biochemistry from the Universidad Nacional Mayor de San Marcos. It was used the Polimerase Chain Reaction (PCR) in order to amplify the region containing the Alu insertion and to determine the presence of TPA-25. The genotype frequency was determined by count of the presence or absence of the Alu insertion and then, evaluated across the test of Chi-square (X2) to know if they were in Hardy-Weinberg's balance. The genotype frequency for the presence of TPA-25 was in Hardy-Weínberg's balance (X2= 1.366, P = 0.505): homocigote 0.108, heterocigote 0.568 and homocigote absence 0.324. In conclusion, in the studied population exists a predominance of the heterocigote TPA-25 over the homocigote TPA- 25.

Downloads

Published

2006-12-29

Issue

Section

Artículos Originales

How to Cite

1.
Casas PM, Zavaleta AI, Cataño HC. Frecuencia genotípica para la presencia de TPA-25 en una población de estudiantes universitarios de Lima-Perú. Ciencia e investigación [Internet]. 2006 Dec. 29 [cited 2024 Jul. 17];9(2):73-5. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5979