Detection of the PrP gene in Junin sheep

Authors

  • Kamilo Rivera J. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Lenin Maturrano H. Laboratorio de Zootecnia y Producción Agropecuaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Juan Manuel Aguilar Instituto de Investigación y Desarrollo de Camélidos Sudamericanos – CONOPA, Lima
  • Raúl Rosadio A. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v26i1.10917

Keywords:

scrapie, Prion gene, Junin sheep

Abstract

The aim of this study was to establish the frequency of resistance and susceptible genotypes to scrapie in the Junin sheep breed. For this, 56 blood samples were collected in a sheep farm in the central highlands of Peru. The DNA was extracted and the PCR products were analyzed by enzymatic digestion using restriction endonucleases that recognize the PrP gene polymorphisms. Four of the 5 haplotypes reported for the PrP gene were identified: ARR (48.2%), ARQ (35.7%), AHQ (10.7%) and VRQ (5.4%). In addition, 7 of the 15 reported genotypes for the Prion gene were found: ARR/ARR (17.9%), ARR/AHQ (10.7%), ARR/ARQ (42.9%), ARQ/AHQ (10.7%), ARQ/ARQ (7.1%), ARR/VRQ (7.1%) and ARQ/VRQ (3.6%). Finally, 82.1% of the animals had genotypes resistant to the disease and 17.9% showed genotypes of susceptibility.

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Published

2015-03-30

Issue

Vol. 26 No. 1 (2015)

Section

Artículos Primarios

License

Copyright (c) 2015 Kamilo Rivera J., Lenin Maturrano H., Juan Manuel Aguilar, Raúl Rosadio A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Rivera J., K., Maturrano H., L., Aguilar, J. M., & Rosadio A., R. (2015). Detection of the PrP gene in Junin sheep. Revista De Investigaciones Veterinarias Del Perú, 26(1), 57-65. https://doi.org/10.15381/rivep.v26i1.10917