Use of seminal plasma in equine epididymal spermatozoa cryopreservation

Authors

  • H. Mauricio Gonzáles Molfino Laboratorio de Biotecnología y Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, Lima
  • Carolina Rodríguez Laboratorio de Biotecnología y Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, Lima
  • Anderson Oropeza Laboratorio de Biotecnología y Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, Lima
  • Yat Sen Wong Laboratorio de Biotecnología y Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, Lima
  • José Llanos Laboratorio de Biotecnología y Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, Lima
  • Hugo Gonzáles Figueroa Laboratorio de Biotecnología y Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Ricardo Palma, Lima

DOI:

https://doi.org/10.15381/rivep.v26i2.11006

Keywords:

sperm, cryopreservation, plasma seminal, epididymis, equine

Abstract

Equine seminal plasma was used in a lactose-EDTA extender for cryopreservation of equine epididymal sperm. Twelve pairs of testicles of slaughtered horses were used. Epididymides were separated and washed applying the retrograde technique to obtain sperm by injecting 10 ml of lactose-EDTA extender through the vas deferens. Samples with more than 30% motility were used. Samples were diluted 1: 1 with the lactose-EDTAextender seminal plasma and filled into 0.5 ml straws at a concentration of 386.3 x 106, frozen in liquid nitrogen and stored for 10 days. The values for fresh and thawed samples were: motility: 43.3 and 16.4% (p<0.05), viability: 48.3 and 40.5%, normal morphology: 67.1 and 56.5%, and integrity of the plasma membrane (HOS): 48.3 and 45.5%.

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Published

2015-06-03

Issue

Vol. 26 No. 2 (2015)

Section

Artículos Primarios

License

Copyright (c) 2015 H. Mauricio Gonzáles Molfino, Carolina Rodríguez, Anderson Oropeza, Yat Sen Wong, José Llanos, Hugo Gonzáles Figueroa

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Gonzáles Molfino, H. M., Rodríguez, C., Oropeza, A., Sen Wong, Y., Llanos, J., & Gonzáles Figueroa, H. (2015). Use of seminal plasma in equine epididymal spermatozoa cryopreservation. Revista De Investigaciones Veterinarias Del Perú, 26(2), 351-356. https://doi.org/10.15381/rivep.v26i2.11006