In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)

Authors

  • Adolfo Alfredo Ochoa Silvera Laboratorio de Parasitología Veterinaria, Escuela de Formación Profesional de Medicina Veterinaria, Universidad Nacional de San Cristóbal de Huamanga, Ayacucho
  • Fidel Rodolfo Mujica Lengua Laboratorio de Biotecnología, Escuela de Formación Profesional de Biología, Universidad Nacional de San Cristóbal de Huamanga, Ayacucho

DOI:

https://doi.org/10.15381/rivep.v26i3.11187

Keywords:

Bacillus thuringiensis, Nematodirus spathiger, biological control

Abstract

A suspension containing the total protein extract of Bacillus thuringiensis of five strains (kurstaki HD-1, thompsoni HD-542, neoleonensis T-24-001, israeliensis HD-500 and israeliensis WHO-2013-9) was obtained. The concentration (mg/ml) of each strain was determined by the Biuret method. The nematicidal activity was evaluated in Petri plates facing eggs and infective larvae (L3) of Nematodirus spathiger against the suspension of total protein extract (1.2 ml). Five treatments (strains) and 10 repetitions were used. The control group contained only the nematodes. The Petri dishes were incubated at 25 °C for 24, 48 and 72 h. Kurstaki HD-1 strain in a concentration of 5.042 mg/ml showed the best anthelmintic efficiency in the three incubation times, obtaining 44.7% mortality in eggs and 45.6% in larva. The total protein extract of B. thuringiensis kurstaki HD-1 contains the nematicidal toxins that could be used for future in vivo experimentally infected animals with N. spathiger.

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RIVEP 26(3), 2015

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Published

2015-09-14

Issue

Vol. 26 No. 3 (2015)

Section

Artículos Primarios

License

Copyright (c) 2015 Adolfo Alfredo Ochoa Silvera, Fidel Rodolfo Mujica Lengua

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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How to Cite

Ochoa Silvera, A. A., & Mujica Lengua, F. R. (2015). In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3). Revista De Investigaciones Veterinarias Del Perú, 26(3), 509-518. https://doi.org/10.15381/rivep.v26i3.11187