A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela

Authors

  • María del Carmen Martínez A. Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana-Alonso”, Universidad de Carabobo (BIOMED-UC)
  • Cruz María Arraga-Alvarado Unidad de Investigaciones Clínicas, Facultad de Ciencias Veterinarias, Universidad del Zulia
  • Francisco Javier Triana-Alonso Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana-Alonso”, Universidad de Carabobo (BIOMED-UC)
  • Johanny Alejandra Ruiz C. Laboratorio de Investigaciones Microbiológicas “Dr. Carlos Palacios”, Departamento de Microbiología, Facultad de Ciencias de la Salud-Sede Aragua, Universidad de Carabobo
  • Clara Nancy Gutiérrez G. Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana-Alonso”, Universidad de Carabobo (BIOMED-UC)

DOI:

https://doi.org/10.15381/rivep.v26i4.11220

Keywords:

Ehrlichia canis, ehrlichiosis, IFI, PCR, Rhipicephalus sanguineus, Venezuela

Abstract

The aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained and analyzed by Indirect Immunofluorescence (IFI) and Nested Polymerase Chain Reaction (PCR). The seroprevalence was 77.3% and the molecular detection was 45.2%. The percentage of dogs infested with ticks was 69%, mostly with Rhipicephalus sanguineus. The results showed that 80% (68/85) of seropositive dogs and 32% (8/25) of seronegative dogs were tick infested (OR=8.5, 95%, CI 3.19 to 22.6; p<0.00001).

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Published

2015-12-31

Issue

Section

Artículos Primarios

How to Cite

Martínez A., M. del C., Arraga-Alvarado, C. M., Triana-Alonso, F. J., Ruiz C., J. A., & Gutiérrez G., C. N. (2015). A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela. Revista De Investigaciones Veterinarias Del Perú, 26(4), 648-656. https://doi.org/10.15381/rivep.v26i4.11220