EFFECT OF TWO FREEZING METHODS ON SPERM VIABILITY OF BOAR SEMEN

Authors

  • Mateo Carpio C. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima
  • José Cadillo C. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima
  • Edwin Mellisho S. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima

DOI:

https://doi.org/10.15381/rivep.v19i1.1172

Keywords:

Boar, semen, cryopreservation, spermatozoa

Abstract

The objective of this experiment was to evaluate the effect of two freezing methods on the spermatic viability of boar semen. Six collects (2 ejaculates per male) of three adult boars (Hampshire, Duroc and Landrace) were used. Immediately after the collection, volume, motility and spermatic concentration of each ejaculate were evaluated. Then, the semen was diluted with BTS solution (Beltsville Thawing Solution) and centrifuged at 1500 rpm for 10 min for plasma withdrawal. The pellet (spermatic portion) was diluted with freezing dilutor (A and B), cooled and equilibrated at 5 °C for two hours before freezing. The equilibrated semen was cryopreserved using two freezing methods: a) in pellets placing 0.25 ml aliquota of semen in holes prepared on the surface of a dry ice block for 20 min and then, pouring them in liquid nitrogen; and b) in straws of 0.5 ml exposing them at 7 cm over liquid nitrogen steam for 10 min (in a styrofoam box). The results showed no statistically differences amongst individual motility and live spermatozoa percentage in semen frozed in pellets (40.1 and 48.8%) as compared to straws (34.5 and 40.7%).

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Published

2008-07-15

Issue

Vol. 19 No. 1 (2008)

Section

Artículos Primarios

License

Copyright (c) 2008 Mateo Carpio C., José Cadillo C., Edwin Mellisho S.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Carpio C., M., Cadillo C., J., & Mellisho S., E. (2008). EFFECT OF TWO FREEZING METHODS ON SPERM VIABILITY OF BOAR SEMEN. Revista De Investigaciones Veterinarias Del Perú, 19(1), 15-19. https://doi.org/10.15381/rivep.v19i1.1172