Development of a PCR assay for the identification of the parasite Dawestrema (Trematoda: Monogenea) in Arapaima gigas fish

Authors

  • Enrique Serrano-Martínez Grupo SALUVET-UPCH, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Manuel Tantaleán V. Grupo SALUVET-UPCH, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Marco Quispe H. Grupo SALUVET-UPCH, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Gina Casas V. Grupo SALUVET-UPCH, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Pablo Londoñe B. Instituto Tecnológico de la Producción, Lima

DOI:

https://doi.org/10.15381/rivep.v27i3.11989

Keywords:

PCR, molecular biology, paiche, parasites, D. cycloancistrium, aquatic health, biodiversity

Abstract

Studies in the Amazonian species Arapaima gigas («paiche») have demonstrated the presence of Dawestrema cycloancistrium monogenean parasite in high parasite loads, mainly in stages between 180 and 365 days of age. D. cycloancistrium adheres to the gills causing a decrease in the degree of oxygenation and stress on the fish predisponing to disease presentation. This study aimed to develop a molecular diagnostic technique for D. cycloancistrium in order to aply it in the management of the fish biodiversity. For this purpose, 200 specimens of D. cycloancistrium were collected, DNA was extracted and quantified, primers were designed and a PCR was developed. The amplification of a sequence of 236 bp of the Dawestrema monogenean was obtained.

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Published

2016-10-01

Issue

Vol. 27 No. 3 (2016)

Section

Artículos Primarios

License

Copyright (c) 2016 Enrique Serrano-Martínez, Manuel Tantaleán V., Marco Quispe H., Gina Casas V., Pablo Londoñe B.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Serrano-Martínez, E., Tantaleán V., M., Quispe H., M., Casas V., G., & Londoñe B., P. (2016). Development of a PCR assay for the identification of the parasite Dawestrema (Trematoda: Monogenea) in Arapaima gigas fish. Revista De Investigaciones Veterinarias Del Perú, 27(3), 581-588. https://doi.org/10.15381/rivep.v27i3.11989