Shigatoxigenic Escherichia coli contamination in chicken meat stalls in a district of Lima, Peru

Authors

  • Juan Raúl Lucas L. Centro de Investigaciones IVITA, Estación El Mantaro, Universidad Nacional Mayor de San Marcos, Huancayo
  • Siever Morales Cauti Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Carlos Eslava Campos Laboratorio de Bacteriología Intestinal, Hospital Infantil Federico Gómez, D.F. México
  • Débora E. Alvarado Laboratorio Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v27i3.12000

Keywords:

Escherichia coli, sale table, cutting board, pathogen, hygiene practices, STEC

Abstract

The aim of this study was to determine if chicken meat stalls are sources of contamination with shigatoxigenic Escherichia coli (STEC) in Lima, Peru. Swabs from the surfaces of hands, cutting boards and sale tables of 50 chicken meat stalls in a large market in the district of San Juan de Miraflores, Lima, Peru were taken (n=150 samples). Standard microbiological isolation and molecular identification of stx1, stx2 and eaeA genes by PCR was performed. Results showed that 42% (63/150) and 25.3% (38/150) of samples were positive for E. coli and STEC respectively. Besides, 84% (42/50) and 66% (33/50) of chicken meat stalls had at least one contaminated surface with E. coli and STEC respectively. Also, 68.3% (43/63) of strains of E. coli isolated were pathogenic for presenting at least one of the evaluated genes. There were 38 STEC strains and presented stx1 (19.0%, 12/63), stx2 (14.3%, 9/63) and the associations stx1 and stx2 (12.7%, 8/63), stx1, stx2 and eaeA (6.3%, 4/63), stx2 and eaeA (4.8%, 3/63), and stx1 y eaeA (3.2%, 2/63). Poor hygiene practices were observed in the chicken meat retail stores. It is confirmed that chicken meat stalls in the Lima market were potential sources of contamination of STEC.

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Published

2016-10-01

Issue

Vol. 27 No. 3 (2016)

Section

Artículos Primarios

License

Copyright (c) 2016 Juan Raúl Lucas L., Siever Morales Cauti, Carlos Eslava Campos, Débora E. Alvarado

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Lucas L., J. R., Morales Cauti, S., Campos, C. E., & Alvarado, D. E. (2016). Shigatoxigenic Escherichia coli contamination in chicken meat stalls in a district of Lima, Peru. Revista De Investigaciones Veterinarias Del Perú, 27(3), 618-625. https://doi.org/10.15381/rivep.v27i3.12000