Effects of two semen extenders on motility and integrity of sperm membrane in ovine frozen semen

Authors

  • Próspero Cabrera V. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima
  • Javier Orellana Ch. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima
  • César Pantoja A. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima

DOI:

https://doi.org/10.15381/rivep.v21i2.130

Keywords:

frozen semen, individual motility, membrane integrity

Abstract

The objective of the study was to evaluate the effects of two semen extenders: Tris- Fructose-egg yolk (Tris) and Citrate-Glucose-egg yolk (citrate) on motility and sperm membrane integrity (HOST) in ovine frozen semen in pellets. The study was carried out at the Semen Bank of the Agrarian University La Molina, in Lima, Peru, using 4 rams (2 Assaf and 2 Blackbelly) of 3.5 to 4 years old. A covariance analysis was used to evaluate the effect of the treatment and breed on Individual Progressive Motility (IPM), and randomized block design to evaluate the effect of extenders on sperm membrane integrity. Semen was frozen of dry ice and thawing was done in test tubes at 38 °C. In the Assaf breed, IPM of thawed semen was 63.77 and 61.11% when using Tris and citrate respectively, showing statistical difference (p<0.05). In the Blackbelly breed the IPM was 62.33 and 61.33%, when used Tris and citrate respectively, and without significant difference. In the Assaf the HOST values of thawed semen were 43.56 and 40.38%, while in Blackbelly were 40.19 and 38.16% respectively, both without significant differences. It is concluded that Tris showed better effect on individual motility but both of them had similar effect on sperm membrane integrity.

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Published

2010-12-31

Issue

Vol. 21 No. 2 (2010)

Section

Artículos Primarios

License

Copyright (c) 2010 Próspero Cabrera V., Javier Orellana Ch., César Pantoja A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Cabrera V., P., Orellana Ch., J., & Pantoja A., C. (2010). Effects of two semen extenders on motility and integrity of sperm membrane in ovine frozen semen. Revista De Investigaciones Veterinarias Del Perú, 21(2), 154-160. https://doi.org/10.15381/rivep.v21i2.130