Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)

Authors

  • Giovanni Restrepo-Betancur Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Medellín
  • Juan David Montoya Páez Facultad de Ciencias Agrarias, Politécnico Colombiano Jaime Isaza Cadavid, Medellín
  • Lucy Arboleda Chacón Facultad de Ciencias Agrarias, Politécnico Colombiano Jaime Isaza Cadavid, Medellín

DOI:

https://doi.org/10.15381/rivep.v28i3.13349

Keywords:

cryopreservation, programmable freezing, fish, spermatozoa, sabaleta

Abstract

The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.05). Sperm vitality was better in the medium velocity curve (52.4 ± 8.6%) in comparison with the ultra-rapid (43.0 ± 19.4%) and slow (29.0 ± 11.8%) curves (p<0.05). EG showed greater sperm vitality (p<0.05). It is concluded that freezing of sabaleta semen (B. henni) by a medium velocity programmable freezing curve and ethylene glycol as cryoprotectant allows better results of post-thaw semen quality.

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Published

2017-10-11

Issue

Vol. 28 No. 3 (2017)

Section

Artículos Primarios

License

Copyright (c) 2017 Giovanni Restrepo-Betancur, Juan David Montoya Páez, Lucy Arboleda Chacón

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Restrepo-Betancur, G., Montoya Páez, J. D., & Arboleda Chacón, L. (2017). Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae). Revista De Investigaciones Veterinarias Del Perú, 28(3), 597-605. https://doi.org/10.15381/rivep.v28i3.13349