Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa

Authors

  • Katherine Choez A. Clínica de Animales Mayores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Luis Ruiz G. Clínica de Animales Mayores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Rocío Sandoval M. Clínica de Animales Mayores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Shirley Evangelista V. Laboratorio de Biotecnologías Reproductivas y Celulares, Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur, Lima
  • Alexei Santiani A. Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v28i3.13367

Keywords:

spermatozoa, epididymis, alpaca, cryopreservation

Abstract

The aim of this study was to determine the optimal concentration of glycerol, ethylene glycol and dimethyl sulfoxide (DMSO) in the cryopreservation of epididymal spermatozoa of alpaca. The effect of different concentrations of glycerol, ethylene glycol and DMSO: T1 (0 M), T2 (0.25 M), T3 (0.5 M), T4 (0.75 M), T5 (1 M), T6 (1.25 M), T7 (1.5 M) and T8 (1.75 M) was evaluated. A polynomial regression model was used to determine the best concentration of each cryoprotectant, resulting 1.16, 1.02 and 0.9 M the optimal concentrations for glycerol, ethylene glycol and DMSO respectively. In Experiment 2, the effect of the optimal concentrations between glycerol, ethylene glycol and DMSO on motility, plasma membrane integrity, and acrosomal viability and integrity in alpaca epididymal spermatozoa were analyzed. The results showed a mean of 35.8, 51.8 and 38.3% for DMSO and 30.8, 45.0 and 33.8% for glycerol for motility, plasma membrane integrity and acrosome viability and integrity, respectively, and without significant differences between these cryoprotectants. However, the results were significantly better (p<0.05) than for ethylene glycol (1.02 M). It is concluded that the concentrations of 0.9 M DMSO and 1.16 M glycerol better preserved the functional characteristics of alpaca epididymal spermatozoa as compared to ethylene glycol (1.02 M).

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Published

2017-10-11

Issue

Vol. 28 No. 3 (2017)

Section

Artículos Primarios

License

Copyright (c) 2017 Katherine Choez A., Luis Ruiz G., Rocío Sandoval M., Shirley Evangelista V., Alexei Santiani A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

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How to Cite

Choez A., K., Ruiz G., L., Sandoval M., R., Evangelista V., S., & Santiani A., A. (2017). Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa. Revista De Investigaciones Veterinarias Del Perú, 28(3), 619-628. https://doi.org/10.15381/rivep.v28i3.13367