Competence of the bovine oocyte obtained by ovum pick-up as evaluated by the bright cresyl blue test

Authors

  • Luis Ayala G. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Jorge Samaniego C. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca https://orcid.org/0000-0002-1599-041X
  • Pedro Nieto E. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Ramiro Rodas C. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Jorge Dutan S. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Guido Calle Or. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Yury Murillo A. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Juan Vazquez M. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Daniel Argudo G. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Fernando Perea G. Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca

DOI:

https://doi.org/10.15381/rivep.v29i2.13816

Keywords:

bovine COC, oocyte morphology, BCB, G6PDH

Abstract

The objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control). The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; however, about 50% of the type B, C and D COCs of T1 and T2 were BCB+. It is concluded that the selection of COC based on morphological characteristics is a reliable method only for type A and has a 50% error for COC type B, C and D and, therefore, the application of the BCB test allows to improve this selection non-invasively.

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Author Biographies

  • Luis Ayala G., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca

    Profesor de Reproduccion y Biotecnologia Animal (PhD), Facultad de Ciencas Agropecuarias

    Director departamente de Postgrados, Facultad de Ciencas Agropecurias

  • Jorge Samaniego C., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Estudiante de Pregrado, Facultad de Ciencias Agropecuarias Medico Veterinario Zootecnista
  • Pedro Nieto E., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Pastos y Forrajes (MSc), Facultad de Ciencas Agropecuarias
  • Ramiro Rodas C., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Fisiologia Animal (MSc), Facultad de Ciencias Agropecuarias
  • Jorge Dutan S., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Histologia (MSc), Facultad de Ciencias Agropecuarias
  • Guido Calle Or., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Clinica Mayor, Facultad de Ciencias Agropecuarias
  • Yury Murillo A., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Laboratorio (MSc), Facultad de Ciencias Agropecuarias
  • Juan Vazquez M., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Patologia Quirurgica (MSc), Facultad de Ciencias Agropecuarias
  • Daniel Argudo G., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Laboratorio de Biotecnologia (MSc), Facultad de Ciencias Agropecuarias
  • Fernando Perea G., Carrera de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
    Profesor de Fisiologia Animal (PhD), Facultad de Ciencias Agropecuarias

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Published

2018-05-31

Issue

Vol. 29 No. 2 (2018)

Section

Artículos Primarios

License

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Ayala G., L., Samaniego C., J., Nieto E., P., Rodas C., R., Dutan S., J., Calle Or., G., Murillo A., Y., Vazquez M., J., Argudo G., D., & Perea G., F. (2018). Competence of the bovine oocyte obtained by ovum pick-up as evaluated by the bright cresyl blue test. Revista De Investigaciones Veterinarias Del Perú, 29(2), 552-558. https://doi.org/10.15381/rivep.v29i2.13816