Freezing of equine semen under two schemes of addition of dimethylformamide

Authors

  • Daniel Domingo Pérez Q. Facultad de Ciencias Agropecuarias, Universidad de La Salle, Bogotá
  • Mariano Acosta L. Facultad de Medicina Veterinaria y Zootecnia, Fundación Universitaria Autónoma de las Américas, Medellín
  • Giovanni Restrepo B. Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Medellín
  • Cesar Camacho Facultade de Ciências Veterinarias, Universidade Federal de Rio Grande do Sul, Porto Alegre
  • Jair Pérez O. Facultad de Ciencias Agropecuarias, Universidad de La Salle, Bogotá

DOI:

https://doi.org/10.15381/rivep.v28i4.13884

Keywords:

extender, sperm quality, cryopreservation, crioprotectant

Abstract

The aim of this study was to evaluate the post-thawing quality of equine semen subjected to two dimethylformamide (DMF) addition schemes during freezing. Semen of five Colombian Creole horses was obtained and diluted in modified INRA82® medium with two DMF addition treatments, T1: immediate addition of 5% DMF, and T2: fractional addition of 5% DMF (during 10 min). Semen was packed into 05. ml straws and subjected to conventional freezing. Sperm motility, vitality and morphology before and after dilution were assessed. In addition, a post-thaw analysis of sperm motility and kinetics using a computerized system and the evaluation of plasma membrane integrity by the hypoosmotic test (HOS) were performed. Before and after freezing, a lower reduction in sperm motility was observed by the immediate addition of DMF (T1), compared to the fractional addition of the cryoprotectant (T2) (p<0.05). Superior values of total and progressive post-thaw motility for T1 were found, while the straightness index (STR) was higher for T2 (p<0.05). It is concluded that the immediate addition of DMF during freezing provides a better protection of the post-thawing sperm motility of stallion semen than the fractional addition of this cryoprotectant.

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Published

2017-12-19

Issue

Vol. 28 No. 4 (2017)

Section

Artículos Primarios

License

Copyright (c) 2017 Daniel Domingo Pérez Q., Mariano Acosta L., Giovanni Restrepo B., Cesar Camacho, Jair Pérez O.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Pérez Q., D. D., Acosta L., M., Restrepo B., G., Camacho, C., & Pérez O., J. (2017). Freezing of equine semen under two schemes of addition of dimethylformamide. Revista De Investigaciones Veterinarias Del Perú, 28(4), 918-927. https://doi.org/10.15381/rivep.v28i4.13884