Freezing of epididymal canine sperm with centrifuged egg yolk

Authors

  • Giovanni Restrepo B. Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Medellín
  • Carlos Andrés Madrid R. Facultad de Medicina Veterinaria y Zootecnia, Universidad CES, Medellín
  • Laura Prieto R. Facultad de Medicina Veterinaria y Zootecnia, Universidad CES, Medellín
  • Juan Esteban Duque C. Facultad de Ciencias Agrarias, Politécnico Colombiano Jaime Isaza Cadavid, Medellín
  • Alexandra Usuga S. Facultad de Medicina Veterinaria y Zootecnia, Universidad CES, Medellín,

DOI:

https://doi.org/10.15381/rivep.v28i4.13886

Keywords:

cryopreservation, extender, spermatozoa, epididymis

Abstract

The aim of this research was to evaluate the use of centrifuged egg yolk (CEY) in the freezing process of canine epididymal spermatozoa. Testicular-epididymis complexes (TECs) were obtained after the orchiectomy of 20 domestic dogs (Canis familiaris) of different breeds and with an age range between 1 and 6 years. The tail of the epididymis was dissected and spermatozoa were obtained by the retrograde flow technique. The spermatozoa were divided into two aliquots and extended in Triladyl® supplemented with 10% CEY (CEY10) or 20% CEY (CEY20). Semen freezing was performed using a standard protocol with liquid nitrogen vapors and semen was packed in straws of 0.5 ml. A computerized system was used to evaluate the motility, kinetics and concentration of spermatozoa. In addition, post-thawing sperm vitality (VE), abnormal morphology (MA) and plasma membrane integrity (HOS) were evaluated. Statistical analysis was performed by adjusting generalized linear models (GLM) and comparing means by the Tukey test. For CEY10 higher values for linear (VSL), curvilinear (VCL) and mean (VAP) velocities were found compared to CEY20 (p<0.05). There were no differences related to the concentration of CEY for other parameters as motility, sperm kinetics, VE, MA and HOS. It is concluded that CEY can be used as supplement for the freezing process of canine epididymal semen. Additionally, when the CEY is used in a proportion of 10% of the extender, it favors the post-thawing sperm kinetics.

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Published

2017-12-19

Issue

Vol. 28 No. 4 (2017)

Section

Artículos Primarios

License

Copyright (c) 2017 Giovanni Restrepo B., Carlos Andrés Madrid R., Laura Prieto R., Juan Esteban Duque C., Alexandra Usuga S.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Restrepo B., G., Madrid R., C. A., Prieto R., L., Duque C., J. E., & Usuga S., A. (2017). Freezing of epididymal canine sperm with centrifuged egg yolk. Revista De Investigaciones Veterinarias Del Perú, 28(4), 876-885. https://doi.org/10.15381/rivep.v28i4.13886