Comparison of the Friedewald formula with the precipitate method for the determination of LDL cholesterol levels in cattle

Authors

  • José Henry Osorio Laboratorio de Investigación en Bioquímica Clínica y Patología Molecular, Departamento de Ciencias Básicas de la Salud, Universidad de Caldas, Manizales
  • Jorge Abel Castañeda Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Manizales

DOI:

https://doi.org/10.15381/rivep.v29i1.14087

Keywords:

LDL cholesterol, cattle, precipitation method, Friedewald formula

Abstract

The aim of this study was to compare two methods (precipitate and Friedewald) for the determination of LDL cholesterol values in cattle. Blood samples were taken from 56 cattle in early morning. In the serum LDL cholesterol levels were determined by the precipitation method and the Friedewald formula. The results were analyzed using a oneway analysis of variance. The precipitated method reported values (mg/dl) of 14.33, 0.36, 59.11, 58.75 and 11.21 for average, minimum, maximum, range and standard deviation, respectively, and by the Friedewald method values (mg/dl) were 62.04, 13.68, 156.82, 143.14 and 32.97 respectively, with significant difference between the two methods for a confidence level of 95.0%. The results indicate that the use of the precipitation method for the determination of LDL cholesterol in cattle is not recommended.

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RIVEP 28(1) 2018

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Published

2018-03-14

Issue

Vol. 29 No. 1 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 José Henry Osorio, Jorge Abel Castañeda

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Osorio, J. H., & Castañeda, J. A. (2018). Comparison of the Friedewald formula with the precipitate method for the determination of LDL cholesterol levels in cattle. Revista De Investigaciones Veterinarias Del Perú, 29(1), 120-125. https://doi.org/10.15381/rivep.v29i1.14087