Effects of cryopreservation on sperm subpopulations in goats

Authors

  • Leonardo Hernández Corredor Unidad de Investigación en Producción Animal (UNIPA), Facultad de Ciencias Veterinarias (FCV), Universidad del Zulia (LUZ), Maracaibo
  • Omar Camargo Rodríguez Universidad Nacional de Colombia, Medellín
  • Albeiro Silva Torres Universidad de Pamplona, Pamplona
  • Juan David Montoya Páez Politécnico Colombiano Jaime Isaza Cadavid, Medellín
  • Armando Quintero Moreno Unidad de Investigación en Producción Animal (UNIPA), Facultad de Ciencias Veterinarias (FCV), Universidad del Zulia (LUZ), Maracaibo

DOI:

https://doi.org/10.15381/rivep.v29i3.14169

Keywords:

spermatozoa, goat, motility, CASA, population, cryopreservation

Abstract

The aim of this study was to evaluate the effect of cryopreservation in the presence of sperm subpopulations, according to motility, in sperm ejaculates using the Computer Assisted Semen Analysis (CASA) system. The motility parameters were analysed with principal component analysis (PCA) where they showed the highest variance, thus reducing the number of variables. In the evaluation of 23 738 fresh spermatozoa, the subpopulation (Sp) 1 consisted of progressive spermatozoa and median progressive motility (18.34%), Sp 2 in high velocity and progressive spermatozoa (20.53%), Sp 3 in high-activity spermatozoa, but not progressive (46.79%) and Sp 4 in little activity and non-progressive spermatozoa (14.32%), showing significant differences in the distribution of the four Sp (p<0.001). The structure of the spermatozoa Sp was not maintained after freezing. When evaluating the same parameters in thawed samples in 36 450 motile sperm, the Sp 1 consisted of slow and linear slow progressive spermatozoa (38.43%), the Sp 2 in slowly active and slow spermatozoa (7.3%), the Sp 3 in spermatozoa with high activity and excellent progressive motility (11.65%) and Sp 4 in active spermatozoa, but not progressive (42.61%), showing significant differences in the distribution of the four Sp (p<0.001). Cryopreservation significantly modified both the specific parameters and the distribution of the spermatozoa within the subpopulations.

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Author Biography

  • Leonardo Hernández Corredor, Unidad de Investigación en Producción Animal (UNIPA), Facultad de Ciencias Veterinarias (FCV), Universidad del Zulia (LUZ), Maracaibo

    Instructor Biotecnologia Reproductiva

    SENA

    Regional Norte de Santander

     

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Published

2018-09-06

Issue

Vol. 29 No. 3 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 leonardo hernández

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Hernández Corredor, L., Camargo Rodríguez, O., Silva Torres, A., Montoya Páez, J. D., & Quintero Moreno, A. (2018). Effects of cryopreservation on sperm subpopulations in goats. Revista De Investigaciones Veterinarias Del Perú, 29(3), 882-893. https://doi.org/10.15381/rivep.v29i3.14169