Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador

Authors

  • Katherine Yuliana Saavedra-Olivos Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Tessy Peralta-Ortiz Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Alberto Ordinola-Zapata Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • John Estuardo Sandoval-Ramayoni Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Enedia Graciela Vieyra-Peña Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Marco Antonio Zapata-Cruz Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Auberto Hidalgo-Mogollón Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Braulio Morán Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Oscar Augusto Mendoza-Neyra Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Magno Ego Mendoza-Dioses Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes
  • Sigrid Yamile Campoverde-Peña Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes, Puerto Pizarro, Tumbes

DOI:

https://doi.org/10.15381/rivep.v29i1.14194

Keywords:

shrimp, AHPND, Litopenaus vannamei, early mortality syndrome, Vibrio parahaemolitycus, PirvpB

Abstract

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible to use MALDI TOF/TOF mass spectrometry in the detection of AHPND-associated proteins in shrimp culture.

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RIVEP 28(1) 2018

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Published

2018-03-14

Issue

Vol. 29 No. 1 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Katherine Yuliana Saavedra-Olivos, Tessy Peralta-Ortiz, Alberto Ordinola-Zapata, John Estuardo Sandoval-Ramayoni, Enedia Graciela Vieyra-Peña, Marco Antonio Zapata-Cruz, Auberto Hidalgo-Mogollón, Braulio Morán, Oscar Augusto Mendoza-Neyra, Magno Ego Mendoza-Dioses, Sigrid Yamile Campoverde-Peña

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How to Cite

Saavedra-Olivos, K. Y., Peralta-Ortiz, T., Ordinola-Zapata, A., Sandoval-Ramayoni, J. E., Vieyra-Peña, E. G., Zapata-Cruz, M. A., Hidalgo-Mogollón, A., Morán, B., Mendoza-Neyra, O. A., Mendoza-Dioses, M. E., & Campoverde-Peña, S. Y. (2018). Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador. Revista De Investigaciones Veterinarias Del Perú, 29(1), 328-338. https://doi.org/10.15381/rivep.v29i1.14194