Immunohistochemical identification of somatostatin and pancreatic polypeptide producing cells in pancreas of alpacas (Vicugna pacos)

Authors

  • Nicanor Suni P. Laboratorio de Anatomía Animal y Fauna Silvestre, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Miluska Navarrete Z. Laboratorio de Anatomía Animal y Fauna Silvestre, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Nieves Sandoval C. Laboratorio de Histología, Embriología y Patología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Francisco Santos R. Laboratorio de Anatomía Animal y Fauna Silvestre, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Alexander Chávez R. Laboratorio de Anatomía Animal y Fauna Silvestre, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Alberto Sato S. Laboratorio de Anatomía Animal y Fauna Silvestre, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v29i1.14199

Keywords:

alpaca, pancreatic polypeptide, somatostatin, pancreas, immunohistochemistry

Abstract

The aim of this study was to identify and evaluate gamma (PP) and delta cells in alpaca pancreas by immunohistochemistry (IHC). Samples were obtained from 30 alpacas from the southern highlands of Peru (crías, juveniles and adults). Histological sections of 5 μm thickness (3 per lobe and 2 of the body) were used. A commercial kit of IHC and polyclonal antibodies (anti-PP and anti-SSTR3) were used to identify PP and delta cells. Rat pancreas samples were used as positive controls. The intensity of the marking was qualitatively classified between 0 (no marking) and 3 (strong marking). PP cells were present in all age groups, mainly in the body and right lobe of the pancreas, being observed mainly in the periphery and intermediate zone of the islets with a medium and strong marking. Delta cells were present in both lobes until 4 months of age, without being observed in juveniles or adults. Delta cells were observed mainly on the periphery of the islets with a light marking.

Downloads

Download data is not yet available.
RIVEP 28(1) 2018

Downloads

Published

2018-03-14

Issue

Vol. 29 No. 1 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Nicanor Suni P., Miluska Navarrete Z., Nieves Sandoval C., Francisco Santos R., Alexander Chávez R., Alberto Sato S.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Suni P., N., Navarrete Z., M., Sandoval C., N., Santos R., F., Chávez R., A., & Sato S., A. (2018). Immunohistochemical identification of somatostatin and pancreatic polypeptide producing cells in pancreas of alpacas (Vicugna pacos). Revista De Investigaciones Veterinarias Del Perú, 29(1), 95-105. https://doi.org/10.15381/rivep.v29i1.14199