Embryonic development capacity of bovine oocytes aspired by ovum pick-up and from slaughterhouse ovaries

Authors

  • Carlos Enrique Quispe Laboratorio de Biotecnología Reproductiva, Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Lima
  • Edith Ancco G. Laboratorio de Biotecnología Reproductiva, Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Lima
  • Juan Solano A. Escuela Profesional de Medicina Veterinaria y Zootecnia, Universidad Peruana Los Andes, Junín
  • Ide Unchupaico P. Facultad de Zootecnia, Universidad Nacional del Centro del Perú, Junín
  • Edwin Mellisho S. Laboratorio de Biotecnología Reproductiva, Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Lima

DOI:

https://doi.org/10.15381/rivep.v29i4.14418

Keywords:

oocytes, blastocysts, embryonic development, ovum pick up

Abstract

The aim of this study was to compare the capacity of embryonic development of oocytes recovered from slaughterhouse ovaries and recovered in vivo by guided ultrasound (ovum pick-up – OPU) in an in vitro production system. Nine sessions in 59 Holstein cows were done to obtain oocytes through OPU and 408 ovaries were collected from 204 cows in the slaughterhouse. For the in vitro maturation of oocytes, only those of grade 1 and 2 were used, matured and fertilized using MIV, FIV and CIV® medium until day 7. The recovery rate of oocytes, grade 1 and 2 oocytes and the percentage of embryos (blastocysts) produced in vitro on day 7 were evaluated. The recovery rate of oocytes collected from slaughterhouse ovaries was higher as compared with oocytes collected by OPU (10.0 vs. 7.2 oocytes per session/cow, p<0.05). There was a greater number of viable oocytes of grade 1 and 2 when the collection was from slaughterhouse ovaries (7.6 vs. 3.4 oocytes per session/cow, p<0.05). The blastocyst rate was 30.5 and 20.8% with slaughterhouse ovarian oocytes and OPU, respectively (p<0.05). A greater capacity to develop oocytes retrieved from slaughterhouse ovaries was obtained than those recovered by ovum pick-up.

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Published

2018-11-25

Issue

Vol. 29 No. 4 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Carlos Enrique Quispe

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Quispe, C. E., Ancco G., E., Solano A., J., Unchupaico P., I., & Mellisho S., E. (2018). Embryonic development capacity of bovine oocytes aspired by ovum pick-up and from slaughterhouse ovaries. Revista De Investigaciones Veterinarias Del Perú, 29(4), 1114-1121. https://doi.org/10.15381/rivep.v29i4.14418