Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia

Authors

  • Pedro Luis Castillo Arroyo Departamento de Ciencias Pecuarias, Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba
  • César Augusto Betancur Hurtado Departamento de Ciencias Pecuarias, Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba
  • Enrique Pardo Pérez Departamento de Biología, Facultad de Ciencias Básicas, Universidad de Córdoba

DOI:

https://doi.org/10.15381/rivep.v29i2.14482

Keywords:

probiotic, cattle, zebu, Enterococcus, antagonistic capacity, stains

Abstract

The objective of the study was to characterize microorganisms with probiotic potential of manure of lactating Brahman calves in Sucre (Colombia). Bacteria and yeasts were isolated from manure samples and the probiotic capacity of these strains was determined by bile salt resistance test (0.05, 0.1, 0.15, 0.3%), resistance to acidic pH (pH 3, 4, 5.6, 7 ), tolerance to NaCl (2, 4, 6, 8, 10%) and antagonistic activity (Salmonella sp and Escherichia coli). Nine microorganisms were identified and three passed the tolerance tests. The antagonistic capacity was determined against pathogenic bacteria (Salmonella sp and E. coli), evidenced by halos (mm) of the three selected strains. The identification of the strains was carried out by the biochemical methods API 50 CHL V5.1 for Lactobacillus and API 20 AUX for yeasts. Molecular analysis of two strains (M13a and 103M2) identified strain M13a as Enterococcus faecium and strain 103M2 as Candida krusei. In conclusion, Enterococcus faecium becomes a viable alternative for the formulation of a biopreparation to improve the productive parameters and decrease gastrointestinal disorders in lactating calves.

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Published

2018-05-31

Issue

Vol. 29 No. 2 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Pedro Luis Castillo Arroyo, César Augusto Betancur Hurtado, Enrique Pardo Pérez

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Castillo Arroyo, P. L., Betancur Hurtado, C. A., & Pardo Pérez, E. (2018). Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia. Revista De Investigaciones Veterinarias Del Perú, 29(2), 438-448. https://doi.org/10.15381/rivep.v29i2.14482