Antimicrobial efficacy of thyme essential oil (Thymus vulgaris) on a Staphylococcus aureus strain

Authors

  • Mayra Montero Recalde Universidad Técnica Ambato, Campus Querochaca, Cevallos, Tungurahua
  • Juan Carlos Mira Universidad Técnica Ambato, Campus Querochaca, Cevallos, Tungurahua
  • Diana Avilés Esquivel Universidad Técnica Ambato, Campus Querochaca, Cevallos, Tungurahua
  • Pilar Pazmiño Miranda Universidad Técnica Ambato, Campus Querochaca, Cevallos, Tungurahua
  • Ramiro Erazo Gutiérrez Universidad Técnica Ambato, Campus Querochaca, Cevallos, Tungurahua

DOI:

https://doi.org/10.15381/rivep.v29i2.14520

Keywords:

minimum bactericidal concentration, minimum inhibitory concentration, MacFarland

Abstract

The objective of this study was to evaluate the in vitro antimicrobial efficacy of thyme essential oil (Thymus vulgaris) on Staphylococcus aureus. Concentrations at 1, 5, 10, 30, 50, 70 and 90% were evaluated in 96.8% dilution in ethanol. The Minimum Inhibitory Concentration was determined by the broth microdilution method. The bacterial inoculum was standardized to 0.5 of the MacFarland scale in spectrophotometer, with the result that the 1% tube of thyme oil did not present turbidity. This, when cultured on Mueller-Hinton agar, determined the Minimum Bactericidal Concentration where no colony growth was observed. The results indicate that treatments at 5 and 10% were not significantly different (p<0.05) with values of inhibition halos of 15.35 mm and 15.9 mm, respetively compared to 1% concentration that presented 12.2 mm of halo of inhibition.

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Published

2018-05-31

Issue

Vol. 29 No. 2 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Mayra Montero Recalde, Juan Carlos Mira, Diana Avilés Esquivel, Pilar Pazmiño Miranda, Ramiro Erazo Gutiérrez

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Montero Recalde, M., Mira, J. C., Avilés Esquivel, D., Pazmiño Miranda, P., & Erazo Gutiérrez, R. (2018). Antimicrobial efficacy of thyme essential oil (Thymus vulgaris) on a Staphylococcus aureus strain. Revista De Investigaciones Veterinarias Del Perú, 29(2), 588-593. https://doi.org/10.15381/rivep.v29i2.14520