Genetic diversity in Asblack sheep (Ovis aries) of Lima, Peru by microsatellite markers

Authors

  • Jonathan Alejandro Morón Barraza "Universidad Nacional Agraria La Molina"
  • C. Yalta Laboratorio de Biología Molecular y Genómica, Dirección de Recursos Genéticos y Biotecnología, Instituto Nacional de Innovación Agraria, Lima
  • G. Gutiérrez Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Lima
  • E. Veli Laboratorio de Biología Molecular y Genómica, Dirección de Recursos Genéticos y Biotecnología, Instituto Nacional de Innovación Agraria, Lima

DOI:

https://doi.org/10.15381/rivep.v30i4.14733

Keywords:

sheep, microsatellites, genetic diversity, population structure

Abstract

The Asblack sheep is the cross of Assaf and Blackbelly to obtain a composite breed. The aim of this study was to determine the genetic diversity of the Asblack and its relationship with the parental breeds. Blood samples were taken from 103 unrelated sheep from the Lima, Peru region. Sheep samples were genotyped for 17 microsatellite markers. High diversity was found in the populations and a total of 146 alleles were detected. Asblack had the highest number of alleles. The expected heterozygosity was greater than the observed in Asblack sheep with regards to Assaf and Blackbelly which indicates a trend for a deficit of heterozygotes in the cross. In addition, nine loci were not in Hardy-Weinberg equilibrium, under the null hypothesis of random union of gametes in the cross. The molecular variation among the populations was 11.4% and the variation among individuals within the populations was 6.81% of the total; however, when analyzing the population structure, the genetic flow was 0.03, which indicates a lower genetic differentiation. The values ​​of FIS (0.077) reflected low levels of inbreeding, and FST (0.115) indicates a moderate genetic differentiation. On the other hand, the populations were separated in the three clusters (K​=3), a result supported by the factorial correspondence analysis. In conclusion, a high genetic diversity was found in the populations studied by breed and individuals, with a moderate differentiation of Asblack sheep with its parental breeds.

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Published

2020-02-04

Issue

Vol. 30 No. 4 (2019)

Section

Artículos Primarios

License

Copyright (c) 2020 Jonathan Alejandro Morón Barraza, C. Yalta, G. Gutiérrez, E. Veli

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Morón Barraza, J. A., Yalta, C., Gutiérrez, G., & Veli, E. (2020). Genetic diversity in Asblack sheep (Ovis aries) of Lima, Peru by microsatellite markers. Revista De Investigaciones Veterinarias Del Perú, 30(4), 1552-1561. https://doi.org/10.15381/rivep.v30i4.14733