Molecular detection of canine distemper virus in clinical cases from unvaccinated domestic dogs and assessment of risk factors

Authors

  • Andrea Soto R. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Luis R. Luna E. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Raúl Rosadio A. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Lenin Maturrano H. Laboratorio de Zootecnia y Producción Agropecuaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v29i3.14744

Keywords:

canine distemper, molecular detection, nucleoprotein, PCR

Abstract

The canine distemper (CD) is a viral infection caused by a Morbillivirus of the family Paramyxoviridae. It can generate digestive, respiratory, and nervous symptomatology, depending on the infecting strain. This study was designed to detect the CD virus through the RT-PCR technique and perform the analysis of the data collected to have a current view of the behavior of this virus in Lima, Peru. Whole blood samples from 52 unvaccinated canines with clinical signs compatible with canine distemper were collected between June 2012 and January 2015. The nucleoprotein of 287 bp was successfully amplified in 32.7% (17/52) of the samples analyzed. In addition, it was found a greater detection of the virus, but not significative, in individuals that manifested systemic clinical signs (respiratory and digestive signs vs nervous) and within the age range of 1.5-4 months.

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Published

2018-09-06

Issue

Vol. 29 No. 3 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Andrea Soto R., Luis R. Luna E., Raúl Rosadio A., Lenin Maturrano H.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Soto R., A., Luna E., L. R., Rosadio A., R., & Maturrano H., L. (2018). Molecular detection of canine distemper virus in clinical cases from unvaccinated domestic dogs and assessment of risk factors. Revista De Investigaciones Veterinarias Del Perú, 29(3), 964-971. https://doi.org/10.15381/rivep.v29i3.14744