Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)

Authors

  • Gustavo Murga A. Laboratorio de Microbiología y Parasitología Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Alberto Manchego S. Laboratorio de Microbiología y Parasitología Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Gina Castro S. Laboratorio de Microbiología y Parasitología Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Danilo Pezo C. Estación Experimental Maranganí, Centro de Investigación IVITA, Universidad Nacional Mayor de San Marcos, Lima
  • Nieves Sandoval C. Laboratorio de Histología, Embriología y Patología Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v29i3.14831

Keywords:

RORγt, interleukin 17, RT-PCR, relative quantification, intestine, alpaca

Abstract

The aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless sex or breed. Samples were taken from the middle segment of the jejunum. The total RNA was extracted, and the RT-PCR technique was used with specific primers. The relative expression of mRNA of RORγt and IL-17 was determined by the 2-ΔΔCt method using two neonatal pups as calibrators and using GAPDH as an endogenous control. The kinetics of expression of RORγt showed a constant trend during the first and third week of life, reaching its maximum expression in animals from 2 to 7 days of age, unlike IL-17 whose expression was progressive with age, evidencing a high stimulation and expression from the first days of age.

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Published

2018-09-06

Issue

Vol. 29 No. 3 (2018)

Section

Artículos Primarios

License

Copyright (c) 2018 Gustavo Murga A., Alberto Manchego S., Gina Castro S., Danilo Pezo C., Nieves Sandoval C.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Murga A., G., Manchego S., A., Castro S., G., Pezo C., D., & Sandoval C., N. (2018). Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos). Revista De Investigaciones Veterinarias Del Perú, 29(3), 1009-1017. https://doi.org/10.15381/rivep.v29i3.14831