Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings

Authors

  • Odalis Epifanía Toledo Valdiviezo Universidad Nacional de Tumbes
  • Arnaldo Castañeda Facultad de Pesquería, Universidad Nacional de Tumbes, Tumbes
  • Manuel A. Feria Facultad de Pesquería, Universidad Nacional de Tumbes, Tumbes
  • Pedro Masías Empresa de Formación e Investigación en Biotecnología Molecular, INCABIOTEC SAC, Tumbes
  • Mario Cueva Cooperativa de Trabajadores Biotecoop, Tumbes
  • Emmerik Motte Empresa de Formación e Investigación en Biotecnología Molecular, INCABIOTEC SAC, Tumbes

DOI:

https://doi.org/10.15381/rivep.v30i3.15400

Keywords:

Arapaima gigas, peptide transporter, PepT1, proteomic, mass spectrometry, MALDI TOF/TOF

Abstract

The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression.

Downloads

Download data is not yet available.

Downloads

Published

2019-10-10

Issue

Vol. 30 No. 3 (2019)

Section

Artículos Primarios

License

Copyright (c) 2019 Odalis Epifanía Toledo Valdiviezo

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Toledo Valdiviezo, O. E., Castañeda, A., Feria, M. A., Masías, P., Cueva, M., & Motte, E. (2019). Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings. Revista De Investigaciones Veterinarias Del Perú, 30(3), 1129-1142. https://doi.org/10.15381/rivep.v30i3.15400