EFECTO DE LA REFRIGERACIÓN SOBRE LA MOTILIDAD, INTEGRIDAD DE MEMBRANA ACROSOMAL Y REACCIÓN ACROSOMAL EN ESPERMATOZOIDES CANINOS

Authors

  • Iris Manosalva P. Laboratorio de Biología de la Reproducción Animal, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos
  • Constanza Cortés Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile
  • Víctor Leyva V. Laboratorio de Reproducción, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos
  • Martha Valdivia C. Laboratorio de Biología de la Reproducción Animal, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos
  • Mónica De los Reyes S. Laboratorio de Reproducción, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile
  • Claudio Barros R. Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile
  • Ricardo Moreno M. Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile

DOI:

https://doi.org/10.15381/rivep.v16i2.1548

Keywords:

dog sperm, acrosome reaction, membrane integrity, mitochondrial activity, chilled sperm

Abstract

Preservation methods of spermatozoa using refrigeration have been developed for canine sperm; however, the rate of fertilization is still low, possibly due to cell damage for unknown reasons. The objective of this study was to evaluate the effect of refrigeration upon motility, mitochondrial activity, acrosomal membrane integrity, and acrosomal reaction in dog sperm incubated in three culture media (Ferp Talp, CCMm, Sp Talp) using new methodologies (Mito Tracker, Lyso Tracker, SBTI). The results indicated that exist a significant decrease (p<0.05) in motility, mitochondrial activity, and acrosomal membrane integrity in refrigerated sperm in comparison with fresh sperm. Also, the dynamic of acrosomal reaction was different in refrigerated sperm in comparison with fresh sperm. The Sp Talp and CCMm media showed the best results in holding the mitochondrial activity and acrosomal membrane integrity.

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Published

2005-12-30

Issue

Vol. 16 No. 2 (2005)

Section

Artículos Primarios

License

Copyright (c) 2005 Iris Manosalva P., Constanza Cortés, Víctor Leyva V., Martha Valdivia C., Mónica De los Reyes S., Claudio Barros R., Ricardo Moreno M.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Manosalva P., I., Cortés, C., Leyva V., V., Valdivia C., M., De los Reyes S., M., Barros R., C., & Moreno M., R. (2005). EFECTO DE LA REFRIGERACIÓN SOBRE LA MOTILIDAD, INTEGRIDAD DE MEMBRANA ACROSOMAL Y REACCIÓN ACROSOMAL EN ESPERMATOZOIDES CANINOS. Revista De Investigaciones Veterinarias Del Perú, 16(2), 114-128. https://doi.org/10.15381/rivep.v16i2.1548