DETERMINACIÓN DE PARENTESCO EN ALPACAS (Vicugna pacos) POR MEDIO DEL ANÁLISIS DE ADN MICROSATÉLITE

Authors

  • Jorge Rodríguez B. CONOPA
  • Jane C. Wheeler CONOPA
  • Ciara S. Dodd Department of Biodiversity and Ecological Processes, Cardiff University, Cardiff, U.K
  • Michael W. Bruford Department of Biodiversity and Ecological Processes, Cardiff University, Cardiff, U.K
  • Raúl Rosadio A. Laboratorio de Microbiología y Parasitología Veterinaria, FMV-UNMSM

DOI:

https://doi.org/10.15381/rivep.v15i2.1579

Keywords:

alpaca, microsatellites, paternity

Abstract

Ten polymorphic microsatellites for alpacas and llamas were used to evaluate paternity in 47 alpacas (18 crias, 18 mothers and 11 fathers) registered at IVITA-Maranganí Research Station, Canchis Province (Cusco-Peru). Analysis was carried out using two methodologies: Automatic Sequencer (ABI 377 DNA sequencers®) and silver staining techniques. Microsatellites were amplified in three multiple and ten single PCR reactions. The number of alleles varied between 4 and 20. The allelic frequencies and the exclusion probability were calculated using Cervus 2.0. All loci, except for two, were within the range published elsewhere. The accumulated exclusion probability for the ten loci was 0.9999. For each multiplex reaction the accumulated exclusion probability was more than 0.90. Both methodologies yielded the same results. The results confirmed paternity in 18 cases of parent-cria pairs, however in 22% of cases (n=4) were identified alternative parents than those indicated in farm records.

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Published

2004-12-31

Issue

Vol. 15 No. 2 (2004)

Section

Artículos Primarios

License

Copyright (c) 2004 Jorge Rodríguez B., Jane C. Wheeler, Ciara S. Dodd, Michael W. Bruford, Raúl Rosadio A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Rodríguez B., J., Wheeler, J. C., Dodd, C. S., Bruford, M. W., & Rosadio A., R. (2004). DETERMINACIÓN DE PARENTESCO EN ALPACAS (Vicugna pacos) POR MEDIO DEL ANÁLISIS DE ADN MICROSATÉLITE. Revista De Investigaciones Veterinarias Del Perú, 15(2), 113-119. https://doi.org/10.15381/rivep.v15i2.1579