Concordance between indirect hemagglutination and enzyme-linked immunosorbent techniques in the diagnosis of porcine toxoplasmosis

Authors

  • Marlene Gonzales L. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Christian Luyo A. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Rosa Pinedo V. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Amanda Chávez V. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Eva Casas A. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v30i1.15668

Keywords:

swine; Toxoplasma gondii; indirect ELISA; HAI

Abstract

The aim of the present study was to determine the concordance between the indirect hemagglutination test (HAI) and the enzyme-linked immunosorbent assay (ELISA) to detect anti-Toxoplasma gondii IgG in the diagnosis of porcine toxoplasmosis. Blood samples were collected from 407 pigs in the finishing phase, from pig farms located in the coastal strip of the department of Lima, Peru. The concordance of the diagnostic techniques and the corresponding seroprevalence were evaluated using the Kappa index and the McNemar test. The results indicated that the concordance between the HAI and ELISA tests through the Kappa index was 26%, considering the regular type, with values of 18.7 ± 3.8% by the HAI method and 14.7 ± 3.4% by the method of ELISA; while no significant differences were found by the McNemar test, which could suggest that both techniques are mutually replaceable. However, it is concluded that, since the correlation is regular, it is not recommended its replacement.

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Published

2019-03-04

Issue

Vol. 30 No. 1 (2019)

Section

Artículos Primarios

License

Copyright (c) 2019 Christian Luyo A., Marlene Gonzales L., Rosa Pinedo V., Amanda Chávez V., Eva Casas A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Gonzales L., M., Luyo A., C., Pinedo V., R., Chávez V., A., & Casas A., E. (2019). Concordance between indirect hemagglutination and enzyme-linked immunosorbent techniques in the diagnosis of porcine toxoplasmosis. Revista De Investigaciones Veterinarias Del Perú, 30(1), 357-363. https://doi.org/10.15381/rivep.v30i1.15668