Genotype identification of Staphylococcus with methicillin-resistant phenotype isolated from human, animal and environmental samples

Authors

  • Luis José Espejo Facultad de Ciencias Agropecuarias, Universidad de La Salle
  • Karen Lorena Rodríguez Facultad de Ciencias Agropecuarias, Universidad de La Salle,
  • Martha Fabiola Rodríguez Facultad de Ciencias de La Salud, Universidad de La Salle
  • Arlen Patricia Gómez Ramírez Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Sede Bogotá https://orcid.org/0000-0001-6460-5162

DOI:

https://doi.org/10.15381/rivep.v30i1.14614

Keywords:

mecA; drug resistance; Staphylococcus spp; veterinary hospital

Abstract

The aim of this study was to identify the mecA-1 gene of Staphylococcus isolates with methicillin-resistant phenotype (SMR) obtained from biological samples and surfaces of a veterinary clinic. Nine isolates with SMR phenotype classified by the automated VITEK system were selected. The genus and the variability were corroborated by amplification and sequencing of the 16S rRNA and tuf genes. The presence of the mecA- 1 gene was also established by PCR. In the nine isolates with SMR phenotype, the 16S rRNA and tuf genes were identified, showing 99% similarity with reference strains; however, only in five isolates was found the mecA-1 gene. The results showed a greater identification of the MR genotype in intra-hospital surfaces, which indicates the need for control and monitoring of these strains in veterinary hospitals due to the implications they have on public health.

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Published

2019-03-04

Issue

Vol. 30 No. 1 (2019)

Section

Artículos Primarios

License

Copyright (c) 2019 Karen Lorena Rodríguez, Luis José Espejo, Martha Fabiola Rodríguez, Arlen Patricia Gómez Ramírez

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Espejo, L. J., Rodríguez, K. L., Rodríguez, M. F., & Gómez Ramírez, A. P. (2019). Genotype identification of Staphylococcus with methicillin-resistant phenotype isolated from human, animal and environmental samples. Revista De Investigaciones Veterinarias Del Perú, 30(1), 364-376. https://doi.org/10.15381/rivep.v30i1.14614