Antimicrobial resistance of Escherichia coli strains isolated from the bursa of Fabricius in broilers

Authors

  • Edna Carvajal B. Universidad de Santander, Facultad de Ciencias de la Salud, Grupo de Investigación CliniUdes, Bucaramanga
  • Walter Hernández A. Universidad de la Paz, Escuela de Medicina Veterinaria y Zootecnia, Barrancabermeja, Colombia
  • María Torres C. Grupo de Investigación de Bacteriología y Laboratorio Clínico, Universidad de Boyacá, Tunja, Boyacá
  • Diana López V. Grupo de Investigación de Bacteriología y Laboratorio Clínico, Universidad de Boyacá, Tunja, Boyacá
  • Egberto Rueda G. Universidad de Santander, Facultad de Ciencias Exactas, Naturales y Agropecuarias, Grupo de investigación en Ciencias Agropecuarias GICA , Bucaramanga
  • María Cristina Vásquez de Díaz Universidad de Santander, Facultad de Ciencias de la Salud, Grupo de Investigación CliniUdes, Bucaramanga

DOI:

https://doi.org/10.15381/rivep.v30i1.14648

Keywords:

antimicrobial resistance; Eschericha coli; broilers

Abstract

The aim of this study was to determine the percentage of sensitivity or resistance of strains of E. coli isolated from asymptomatic broiler chicken against 18 common antibiotics used in poultry and in human medicine. In addition, strains that produce extended-spectrum beta-lactamases (ESBL) from a farm or slaughterhouses in the poultry area of Santander, Colombia. The circulation of strains resistant to beta-lactams, cephalosporins, aminoglycosides, quinolones and sulfonamides was determined, as well as antibiotics widely used in other non-farm environments. The content of the bursa of Fabricius was cultivated in McConkey agar obtaining 46 strains of E. coli. Susceptibility tests (n=18) were conducted to the isolated strains, and 91% of the strains were resistant to ampicillin and 80% to cephalosporins. The association of antibiotics with beta-lactamase inhibitors (ampicillin sulbactam and amoxicillin / clavulanic acid) showed 30% resistance. The double disc test was used to evaluate the presence of strains of E. coli producing extendedspectrum beta-lactamases (ESBL) confirming its presence in 63% of the samples. Results showed the presence of E. coli strains in asymptomatic chicken broilers with high antimicrobial resistance, including expression of ESBL.

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Author Biography

  • María Cristina Vásquez de Díaz, Universidad de Santander, Facultad de Ciencias de la Salud, Grupo de Investigación CliniUdes, Bucaramanga

    Bacteriologa y Laboratorista Clìnico, Maestria en Biotecnologia avanzada, docente investigadora , Directora del programa de Bacteriologìa y Laboratorio Clìnico de la Facultad de Ciencias de la Salud

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Published

2019-03-04

Issue

Vol. 30 No. 1 (2019)

Section

Artículos Primarios

License

Copyright (c) 2019 Walter Hernández A., Edna Carvajal B., María Torres C., Diana López V., Egberto Rueda G., María Cristina Vásquez de Díaz

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Carvajal B., E., Hernández A., W., Torres C., M., López V., D., Rueda G., E., & Vásquez de Díaz, M. C. (2019). Antimicrobial resistance of Escherichia coli strains isolated from the bursa of Fabricius in broilers. Revista De Investigaciones Veterinarias Del Perú, 30(1), 430-437. https://doi.org/10.15381/rivep.v30i1.14648