Phenotypic detection of antimicrobial resistance mechanisms of Escherichia coli isolates from enteric infections in pigs from technified farms

Authors

  • Michelle Monterroso C. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Guillermo Salvatierra R. Universidad San Ignacio de Loyola, Lima
  • André Sedano S. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Sonia Calle E. Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v30i1.15670

Keywords:

Escherichia coli; pigs; resistance mechanisms; antimicrobial resistance; public health

Abstract

The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6’), 28% (10/36) to ANT (2") and 11% (4/36) to AAC (3) IV.

Downloads

Download data is not yet available.

Downloads

Published

2019-03-04

Issue

Vol. 30 No. 1 (2019)

Section

Artículos Primarios

License

Copyright (c) 2019 Guillermo Salvatierra R., Michelle Monterroso C., André Sedano S., Sonia Calle E.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Monterroso C., M., Salvatierra R., G., Sedano S., A., & Calle E., S. (2019). Phenotypic detection of antimicrobial resistance mechanisms of Escherichia coli isolates from enteric infections in pigs from technified farms. Revista De Investigaciones Veterinarias Del Perú, 30(1), 455-464. https://doi.org/10.15381/rivep.v30i1.15670