Serum leptin levels in poultry

Authors

  • José Henry Osorio Laboratorio de Investigación en Bioquímica Clínica y Patología Molecular, Departamento de Ciencias Básicas de la Salud, Universidad de Caldas, Manizales
  • Jorge Abel Castañeda Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Manizales

DOI:

https://doi.org/10.15381/rivep.v30i2.16073

Keywords:

adipocytokines; birds; immunoassay; leptin; metabolism

Abstract

The aim of this study was to determine the levels of leptin in broilers and laying hens by using a kit designed for human samples. Fasting blood samples were collected from 80 broilers of the Cobb 500 line of 4 weeks of age (40 females and 40 males) and 86 hens of 26 weeks of age (43 Lohmann Brown and 43 Hy-line). Leptin levels were determined by an ELISA sandwich, after validation of the kit for use in commercial poultry. The mean, minimum, maximum and standard deviation values ​​of leptin for broilers were 0.61, 0.12, 3.99 and 0.10 ng/ml, respectively, without significant difference between sexes. Laying hens reported a mean, minimum, maximum and standard deviation values ​​of 0.66, 0.16, 1.83 and 0.11 ng/ml, respectively, without significant difference between lines. Similarly, no statistical difference was found between broiler chickens and laying hens. The results of the study indicate that the reference values for leptin in chickens and chickens are 0.63 ng/ml, and the levels can be measured by using the human kit.

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Published

2019-07-04

Issue

Vol. 30 No. 2 (2019)

Section

Artículos Primarios

License

Copyright (c) 2019 José Henry Osorio, Jorge Abel Castañeda

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Osorio, J. H., & Castañeda, J. A. (2019). Serum leptin levels in poultry. Revista De Investigaciones Veterinarias Del Perú, 30(2), 598-604. https://doi.org/10.15381/rivep.v30i2.16073