Detection of tilapia lake virus (TiLV) by seminested RT-PCR in farmed tilapias from two regions of Peru

Authors

  • Arnaldo Edward Castañeda Vargas Incabiotec, Tumbes, Perú
  • Manuel Manuel Feria Incabiotec, Tumbes, Perú
  • Odalis Odalis Toledo Incabiotec, Tumbes, Perú
  • Deysy Castillo Cooperativa de Trabajadores Biotecoop, Tumbes, Perú
  • Mario Cueva Cooperativa de Trabajadores Biotecoop, Tumbes, Perú
  • E. Motte Incabiotec, Tumbes, Perú

DOI:

https://doi.org/10.15381/rivep.v31i2.16158

Keywords:

tilapia lake virus, PCR, tilapia, TiLV

Abstract

The production of tilapia (Oreochromis sp) is being affected by outbreaks of lake tilapia virus (TiLV) with high mortality in the main breeding regions in Peru. The aim of this study was to determine the presence of TiLV in two tilapia fish farms in Piura and San Martín, which presented mortalities greater than 60%. Samples (liver and brain) were taken from 70 fish from different stages of culture that showed lethargy, loss of appetite, eye lesions and skin erosions. The detection and confirmation of TiLV was determined by semi-nested RT-PCR and subsequent sequencing of the amplified product of segment 3 of the virus. Products of the expected weight (250 bp) corresponding to samples from the two production centres were found. The phylogenetic analysis showed a high homology among the isolates and an identity greater than 90% with the Israeli reference strain (KU751816.1).

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Published

2020-06-20

Issue

Vol. 31 No. 2 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Arnaldo Edward Castañeda Vargas, Manuel Manuel Feria, Odalis Odalis Toledo, Deysy Castillo, Mario Cueva, E. Motte

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Castañeda Vargas, A. E., Manuel Feria, M., Odalis Toledo, O., Castillo, D., Cueva, M., & Motte, E. (2020). Detection of tilapia lake virus (TiLV) by seminested RT-PCR in farmed tilapias from two regions of Peru. Revista De Investigaciones Veterinarias Del Perú, 31(2), e16158. https://doi.org/10.15381/rivep.v31i2.16158