COMPARACIÓN DE UNA PRUEBA DE ELISA ESTÁNDAR Y ELISA DE RANGO EXTENDIDO PARA LA ENFERMEDAD INFECCIOSA DE LA BURSA EN AVES

Authors

  • Magali Salas M. Práctica Privada.
  • Eliana Icochea D’A. Laboratorio de Patología Aviar y Producción Avícola, FMV-UNMSM
  • César Gavidia Ch. Laboratorio de Medicina Veterinaria Preventiva, FMV-UNMSM

DOI:

https://doi.org/10.15381/rivep.v13i1.1708

Keywords:

Infectious Bursal Disease virus (IBDV), antibodies, ELISA Standard (ELISA IBD), ELISA of Extended Range (ELISA IBD-XR)

Abstract

A total of 180 paired serum samples from 3 breeder groups (broiler’s grandparents and parents), and layers) were used to detect antibodies against the Infectious Bursal Disease virus through the ELISA test. Animals were tested at three ages (1-3 days, 16, and 27 weeks of age). Two commercial kits were tested: the standard ELISA (ELISA-IBD) and the new named Extended Range ELISA (ELISA-XR). The latter detected higher titres of antibodies than the ELISA-IBD. Broiler’s parents had the higher levels of antibodies indicating better immune response than the other two groups. The titre distribution had a more uniform distribution using the ELISA-IBD rather that the ELISA-XR; however all titres were above the minimum accepted ranges when the ELISA-XR was used (p<0.05). The results indicated that the new ELISA-XR was more efficient in detecting antibodies against the Infectious Bursal Disease virus.

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Published

2002-07-15

Issue

Vol. 13 No. 1 (2002)

Section

Artículos Primarios

License

Copyright (c) 2002 Magali Salas M., Eliana Icochea D’A., César Gavidia Ch.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Salas M., M., Icochea D’A., E., & Gavidia Ch., C. (2002). COMPARACIÓN DE UNA PRUEBA DE ELISA ESTÁNDAR Y ELISA DE RANGO EXTENDIDO PARA LA ENFERMEDAD INFECCIOSA DE LA BURSA EN AVES. Revista De Investigaciones Veterinarias Del Perú, 13(1), 67-71. https://doi.org/10.15381/rivep.v13i1.1708