Molecular identification of Flavobacterium psychrophilum and characterization of cutaneous alterations in rainbow trout (Oncorhynchus mikyss) from Puno, Peru

Authors

  • Carlos Távara P. Grupo SANIVET, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Enrique Serrano-Martínez Grupo SANIVET, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Carolina Tafalla Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid
  • Cesar Burga C. Grupo SANIVET, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Luis Llanco A. Grupo SANIVET, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima

DOI:

https://doi.org/10.15381/rivep.v30i4.17149

Keywords:

Oncorhynchus mikyss, PCR, Flavobacterium psychrophilum, histology

Abstract

The aim of this study was to compare histological lesions of rainbow trout (Oncorhynchus mikyss) affected by the bacterial cold water disease (BCDW) with asymptomatic trout skin samples from fish farms in Lake Titicaca (Puno, Peru) and identify the presence of Flavobacterium psychrophilum by conventional PCR. Thirteen trout with skin lesions and seven without lesions, with sizes greater than 25 cm and average weight of 350 g were used. Conventional PCR was performed for the identification of Flavobacterium psychrophilum, using primers directed to the 16S rRNA region. Molecularly, both groups of animals were positive for the presence of F. psycrophilum. It can be concluded that this pathogen is present in the southern part of the country.

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Published

2020-02-04

Issue

Vol. 30 No. 4 (2019)

Section

Artículos Primarios

License

Copyright (c) 2020 Carlos Távara P., Enrique Serrano-Martínez, Carolina Tafalla, Cesar Burga C., Luis Llanco A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Távara P., C., Serrano-Martínez, E., Tafalla, C., Burga C., C., & Llanco A., L. (2020). Molecular identification of Flavobacterium psychrophilum and characterization of cutaneous alterations in rainbow trout (Oncorhynchus mikyss) from Puno, Peru. Revista De Investigaciones Veterinarias Del Perú, 30(4), 1762-1769. https://doi.org/10.15381/rivep.v30i4.17149