Addition of cholesterol-loaded methyl-β-cyclodextrin in cryopreservation of ram semen

Authors

  • Luis Antonio Castillo Cevallos Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Lima
  • Enrique Páucar Espinoza Banco Nacional de Semen, Universidad Nacional Agraria La Molina, Lima
  • Enrique Alvarado Malca Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina, Lima

DOI:

https://doi.org/10.15381/rivep.v30i4.17160

Keywords:

cryopreservation, semen, cyclodextrin, cholesterol, ram

Abstract

The effect of the addition of cholesterol-loaded methyl-β-cyclodextrin (CLC) on the microscopic characteristics of chilled and frozen/thawed ram semen was evaluated. Four 1-2-year-old Asblack rams were used. Thirty ejaculates divided into groups of three aliquots each were used to which CLC (0, 1 and 2 mg of CLC/120 million sperm, T1, T2 and T3 respectively) were added at 22 °C and incubated for 15 minutes. Samples were then refrigerated and stabilized at 5 °C for 18 hours. Semen freezing was done in straws using nitrogen vapours for 4 minutes (-120 °C) and then submerged in liquid nitrogen. There were no statistical differences between treatment averages in all characteristics of refrigerated semen. The post thawing results showed improvements with the use of cholesterol in the cryopreservation of ram semen (p<0.5), obtaining a motility of 65.3, 68.7 and 75.37%, vitality of 56.6, 60.7 and 66.2% and membrane integrity of 50.5, 56.4 and 63.7% in T1, T2 and T3 respectively. There was no statistical difference between treatments with respect to sperm abnormalities.

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Published

2020-02-04

Issue

Vol. 30 No. 4 (2019)

Section

Artículos Primarios

License

Copyright (c) 2020 Luis Antonio Castillo Cevallos, Enrique Páucar Espinoza, Enrique Alvarado Malca

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Castillo Cevallos, L. A., Páucar Espinoza, E., & Alvarado Malca, E. (2020). Addition of cholesterol-loaded methyl-β-cyclodextrin in cryopreservation of ram semen. Revista De Investigaciones Veterinarias Del Perú, 30(4), 1637-1644. https://doi.org/10.15381/rivep.v30i4.17160