Addition of cholesterol-loaded methyl-β-cyclodextrin in cryopreservation of ram semen
DOI:
https://doi.org/10.15381/rivep.v30i4.17160Keywords:
cryopreservation, semen, cyclodextrin, cholesterol, ramAbstract
The effect of the addition of cholesterol-loaded methyl-β-cyclodextrin (CLC) on the microscopic characteristics of chilled and frozen/thawed ram semen was evaluated. Four 1-2-year-old Asblack rams were used. Thirty ejaculates divided into groups of three aliquots each were used to which CLC (0, 1 and 2 mg of CLC/120 million sperm, T1, T2 and T3 respectively) were added at 22 °C and incubated for 15 minutes. Samples were then refrigerated and stabilized at 5 °C for 18 hours. Semen freezing was done in straws using nitrogen vapours for 4 minutes (-120 °C) and then submerged in liquid nitrogen. There were no statistical differences between treatment averages in all characteristics of refrigerated semen. The post thawing results showed improvements with the use of cholesterol in the cryopreservation of ram semen (p<0.5), obtaining a motility of 65.3, 68.7 and 75.37%, vitality of 56.6, 60.7 and 66.2% and membrane integrity of 50.5, 56.4 and 63.7% in T1, T2 and T3 respectively. There was no statistical difference between treatments with respect to sperm abnormalities.
Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2020 Luis Antonio Castillo Cevallos, Enrique Páucar Espinoza, Enrique Alvarado Malca
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
AUTHORS RETAIN THEIR RIGHTS:
a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.
b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).
c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).