Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017

Authors

  • Rony Yunior Cotaquispe Nalvarte Laboratorio Bioservice SRL, Lima
  • Jorge Rodríguez Bailón Laboratorio Bioservice SRL, Lima
  • Isabel Koga Yanagui Laboratorio Bioservice SRL, Lima
  • Robert Tinoco Romero Laboratorio Bioservice SRL, Lima
  • Irene De La Flor Montaubán Unidad de Biotecnología Molecular, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima
  • Alberto Manchego Sayán Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v30i4.17186

Keywords:

Ornithobacterium rhinotracheale, antibiotic resistance, genotyping, ERIC-PCR

Abstract

Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin.

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Published

2020-02-04

Issue

Vol. 30 No. 4 (2019)

Section

Artículos Primarios

License

Copyright (c) 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayán

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

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How to Cite

Cotaquispe Nalvarte, R. Y., Rodríguez Bailón, J., Koga Yanagui, I., Tinoco Romero, R., De La Flor Montaubán, I., & Manchego Sayán, A. (2020). Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017. Revista De Investigaciones Veterinarias Del Perú, 30(4), 1734-1742. https://doi.org/10.15381/rivep.v30i4.17186