Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico

Authors

  • Enrique Salazar Grosskelwing Facultad de Medicina Veterinaria y Zootecnia, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán
  • Manuel Emilio Bolio González Facultad de Medicina Veterinaria y Zootecnia, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán
  • Roger Iván Rodríguez Vivas Facultad de Medicina Veterinaria y Zootecnia, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán
  • Guadalupe Miró Corrales Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid
  • Francisco Javier Escobedo Ortegón Laboratorio de Zoonosis y Otras Enfermedades Transmitidas por Vectores, Centro de Investigaciones Regionales “Hideyo Noguchi”, Universidad Autónoma de Yucatán
  • Hugo Antonio Ruíz Piña Laboratorio de Zoonosis y Otras Enfermedades Transmitidas por Vectores, Centro de Investigaciones Regionales “Hideyo Noguchi”, Universidad Autónoma de Yucatán
  • Elsy Nalleli Loría Cervera Laboratorio de Inmunología, Centro de Investigaciones Regionales “Hideyo Noguchi”, Universidad Autónoma de Yucatán

DOI:

https://doi.org/10.15381/rivep.v31i4.17336

Keywords:

dogs, indirect immunofluorescence, Leishmania spp, PCR, trypanosomatids, Trypanosoma cruzi

Abstract

A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection by T. cruzi (42.5%) and none for infection by Leishmania spp. Dogs with low body condition showed greater seropositivity to T. cruzi using Fisher’s exact test (p<0.05). Parasites of the genus Leishmania did not actively participate in the pathological processes of the animals; however, a high frequency of animals infected with T. cruzi was observed.

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Published

2020-11-24

Issue

Vol. 31 No. 4 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Enrique Salazar Grosskelwing, Manuel Emilio Bolio González, Roger Iván Rodríguez Vivas, Guadalupe Miró Corrales, Francisco Javier Escobedo Ortegón, Hugo Antonio Ruíz Piña, Elsy Nalleli Loría Cervera

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How to Cite

Salazar Grosskelwing, E., Bolio González, M. E., Rodríguez Vivas, R. I., Miró Corrales, G., Escobedo Ortegón, F. J., Ruíz Piña, H. A., & Loría Cervera, E. N. (2020). Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico. Revista De Investigaciones Veterinarias Del Perú, 31(4), e17336. https://doi.org/10.15381/rivep.v31i4.17336