The time interval between ovarian stimulation with FSH/LH and ovum-pick up affects the quantity, quality and developmental competence of oocytes retrieved from Ecuadorian creole heifers

Authors

  • Luis Ayala Guanga Carrera de Medicina Veterinaria Zootecnia, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Jorge Samaniego Campoverde Laboratorio de Biotecnología, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Daniel Argudo Garzón Carrera de Medicina Veterinaria Zootecnia, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Mariana Perea Brugal Facultad de Ciencias Veterinarias, Universidad del Zulia
  • Fernando Perea Ganchou Carrera de Medicina Veterinaria Zootecnia, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Ermes Rodas Carpio Carrera de Medicina Veterinaria Zootecnia, Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Pedro Nieto Escandón Carrera de Medicina Veterinaria Zootecnia, Facultad de Ciencias Agropecuarias, Universidad de Cuenca

DOI:

https://doi.org/10.15381/rivep.v31i1.17571

Keywords:

COCs, ovarian stimulation, follicular aspiration, Brillant Cresyl Blue

Abstract

The aim of this study was to evaluate the effects of gonadotropin treatment and the interval between ovarian stimulation with FSH/LH and the follicular aspiration session, on the quantity and quality of the cumulus oocyte complexes (COCs) obtained by ovum pick up (OPU) ) in Ecuadorian Creole heifers. Four heifers were randomly assigned to four groups (FHS/LH 40h; FSH/LH 48h; control 40h; control 48h). In two groups a dose (0.011 mg, IM) of GnRH was applied on day 0, followed by 500 IU, IM, of FSH/LH on day 2, and the OPU session at 40 (FSH/LH 40 h) or 48 hours (FSH/LH 48h) after the application of gonadotropin. In the other two groups, FSH/LH was replaced by a physiological solution. There was no statistical difference in the recovery rate of COCs between FSH/LH 40h and FSH/LH 48h, but approximately twice as many COCs (p<0.05) were obtained than in those who did not receive ovarian stimulation. In addition, 2.4 times more COCs of quality A were achieved in the treatment of FSH/LH 48h than in that of FSH/LH 40h. All (100%) of these structures reacted positively to Brilliant Cresyl Blue (BCB+), which means that they finished the growth and were competent to initiate in vitro maturation. It is concluded that the treatment with FSH/LH 48h allowed to obtain a greater number of COCs competent for in vitro maturation.

Downloads

Download data is not yet available.

Downloads

Published

2020-03-29

Issue

Vol. 31 No. 1 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Luis Ayala Guanga, Jorge Samaniego Campoverde, Daniel Argudo Garzón, Mariana Perea Brugal, Fernando Perea Ganchou, Ermes Rodas Carpio, Pedro Nieto Escandón

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Ayala Guanga, L., Samaniego Campoverde, J., Argudo Garzón, D., Perea Brugal, M., Perea Ganchou, F., Rodas Carpio, E., & Nieto Escandón, P. (2020). The time interval between ovarian stimulation with FSH/LH and ovum-pick up affects the quantity, quality and developmental competence of oocytes retrieved from Ecuadorian creole heifers. Revista De Investigaciones Veterinarias Del Perú, 31(1), e17571. https://doi.org/10.15381/rivep.v31i1.17571