Evaluation of three concentration of centrifuged egg yolk in the cryopreservation of bovine semen

Authors

  • Juan David Montoya-Páez Facultad de Ciencias Agrarias, Politécnico Colombiano Jaime Isaza Cadavid, Medellín, Colombia
  • Mariana Giraldo-León Facultad de Ciencias Agrarias, Politécnico Colombiano Jaime Isaza Cadavid, Medellín, Colombia
  • Juan Esteban Duque-Cortes Facultad de Ciencias Agrarias, Politécnico Colombiano Jaime Isaza Cadavid, Medellín, Colombia

DOI:

https://doi.org/10.15381/rivep.v31i2.17818

Keywords:

seminal quality, spermatozoa, centrifugation, freezing

Abstract

The aim of this study was to evaluate three concentrations of centrifuged egg yolk in a commercial diluent for bovine semen. Ejaculates from four bulls were obtained and semen collections were made by electroejaculation and artificial vagina. The sperm obtained were divided into three aliquots supplemented with 10, 20 and 30% centrifuged egg yolk (YHC). Semen freezing was done in 0.5 ml straws. Thawed semen was evaluated for total (MT) and progressive motility (MP), vitality (VE), abnormal morphology (MA) and plasma membrane integrity (MI). Statistical analysis was performed by adjusting generalized linear models (GLM) and comparing means by the Tukey test. No significant differences were found for MT, VE, MA and IM between treatments, but there were significant differences for treatment with 10% YHC supplementation for linear (VSL), curvilinear (VCL) and mean (VAP) speeds with respect to 20 and 30% of YHC (p<0.05). It is concluded that using 10% YHC for cryopreservation of bovine semen improves the velocity of the spermatozoa and favours the preparation of the extender for cryopreservation of cells.

Downloads

Download data is not yet available.

Downloads

Published

2020-06-20

Issue

Vol. 31 No. 2 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Juan David Montoya-Páez, Mariana Giraldo-León, Juan Esteban Duque-Cortes

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Montoya-Páez, J. D., Giraldo-León, M., & Duque-Cortes, J. E. (2020). Evaluation of three concentration of centrifuged egg yolk in the cryopreservation of bovine semen. Revista De Investigaciones Veterinarias Del Perú, 31(2), e17818. https://doi.org/10.15381/rivep.v31i2.17818