Desarrollo de una técnica de PCR Development of a PCR technique for the detection of Fowl adenovirus 4 (FADV-4) in tissue samples

Authors

  • Víctor Chávez-Montenegro Laboratorios de Investigación y Desarrollo de Farmacológicos Veterinarios (FARVET S.A.C.) https://orcid.org/0000-0003-3554-6292
  • Vladimir Longa-Bobadilla Laboratorios de Investigación y Desarrollo de Farmacológicos Veterinarios (FARVET S.A.C.)
  • Phillip Ormeño-Vásquez Laboratorios de Investigación y Desarrollo de Farmacológicos Veterinarios (FARVET S.A.C.)
  • Luis Tataje Lavanda Laboratorios de Investigación y Desarrollo de Farmacológicos Veterinarios (FARVET S.A.C.)
  • Manolo Fernández Díaz Laboratorios de Investigación y Desarrollo de Farmacológicos Veterinarios (FARVET S.A.C.)

DOI:

https://doi.org/10.15381/rivep.v32i2.18110

Keywords:

FAdV-4, PCR, primers, standardization, sensitivity, specificity

Abstract

            It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.

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Author Biography

  • Víctor Chávez-Montenegro, Laboratorios de Investigación y Desarrollo de Farmacológicos Veterinarios (FARVET S.A.C.)

    I am a biologist graduated and titled from the UNIVERSIDAD NACIONAL DE PIURA, belonging to the Colegio de Biólogos del Perú with the code C.B.P N° 11452 with updated SKILLS. During my professional stage I have had labor experience in different areas of laboratory as:
    - FORENSE BIOLOGY LABORATORY, working in the Ministerio Público de la División Médico Legal de Lima Norte, from March of this year until now.
    - CLINICAL AND MICROBIOLOGICAL LABORATORY and ENVIRONMENTAL HEALTH LABORATORY working in the Ministry of Health (MINSA) for more than four and a half years - including SERUMS.
    - MOLECULAR AND GENOMIC BIOTECHNOLOGY LABORATORY in the private company FARVET S.A.C. for seven (07) months.
    I am characterized by being proactive, responsible, punctual, teamwork, analytical capacity and constructive critical sense, with personal and institutional values, work under pressure, effective communication and leadership. My objective is to provide solutions to the problems that most afflict the Institution.

    Translated with www.DeepL.com/Translator

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Published

2021-04-23

Issue

Vol. 32 No. 2 (2021)

Section

Artículos Primarios

License

Copyright (c) 2021 Víctor Chávez-Montenegro, Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Luis Tataje Lavanda, Manolo Fernández Díaz

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Chávez-Montenegro, V., Longa-Bobadilla, V., Ormeño-Vásquez, P., Tataje Lavanda, L., & Fernández Díaz, M. (2021). Desarrollo de una técnica de PCR Development of a PCR technique for the detection of Fowl adenovirus 4 (FADV-4) in tissue samples. Revista De Investigaciones Veterinarias Del Perú, 32(2), e18110. https://doi.org/10.15381/rivep.v32i2.18110