Effect of two fertilization medium on the in vitro development of Creole bovine embryosne embryos

Authors

  • José Goicochea Vargas Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0002-3938-1563
  • Wilson Rondón Jorge Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0003-1080-4910
  • Fidel Acosta Pachorro Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0002-1378-9886
  • Yusep Gómez Marín Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0001-9740-6047
  • Magaly Montalvo Martin Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0002-0898-103X
  • Max Salvatierra Alor Laboratorio de Biotecnología Molecular, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0002-8190-9288
  • Jéssica Martel Falcón Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0003-3210-3795
  • Oscar Ballarte Zevallos Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0001-7440-1614
  • Julio Díaz Zegarra Laboratorio de Cirugía y Biotecnología Reproductiva, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan (UNHEVAL), Huánuco, Peru https://orcid.org/0000-0003-3056-3763
  • Marcelo Ratto Fuster Instituto de Ciencia Animal, Universidad Austral de Chile, Valdivia, Chile

DOI:

https://doi.org/10.15381/rivep.v32i5.18709

Keywords:

FERT-TALP, TCM-199, in vitro, embryos, native cattle

Abstract

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while none wwere achieved with the modified TCM-199 medium.

Downloads

Download data is not yet available.

Downloads

Published

2021-10-27

Issue

Vol. 32 No. 5 (2021)

Section

Artículos Primarios

License

Copyright (c) 2021 José Goicochea Vargas, Wilson Rondón Jorge, Fidel Acosta Pachorro, Yusep Gómez Marín, Magaly Montalvo Martin, Max Salvatierra Alor, Jéssica Martel Falcón, Oscar Ballarte Zevallos, Julio Díaz Zegarra, Marcelo Ratto Fuster

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Goicochea Vargas, J., Rondón Jorge, W., Acosta Pachorro, F., Gómez Marín, Y., Montalvo Martin, M., Salvatierra Alor, M., Martel Falcón, J., Ballarte Zevallos, O., Díaz Zegarra, J., & Ratto Fuster, M. (2021). Effect of two fertilization medium on the in vitro development of Creole bovine embryosne embryos. Revista De Investigaciones Veterinarias Del Perú, 32(5), e18709. https://doi.org/10.15381/rivep.v32i5.18709