Genotyping and phenotyping of Gallibacterium sp from poultry with respiratory clinical signs

Authors

  • Rony Yunior Cotaquispe Nalvarte Laboratorio de Biología Molecular, Bioservice SRL, Lima
  • Ysabel Koga Yanagui Laboratorio de Microbiología, Bioservice SRL, Lima
  • Robert Tinoco Romero Laboratorio de Microbiología, Bioservice SRL, Lima

DOI:

https://doi.org/10.15381/rivep.v31i4.19019

Keywords:

Gallibacterium sp, phenotyping, genotyping, ERIC-PCR, REP-PCR, BOXAIR

Abstract

The aim of this study was the phenotyping of 60 isolates of Gallibacterium sp through a profile of six families of antibiotics and their genotyping by ERIC-PCR, REP-PCR and BOXAIR techniques. It was found that 98.3% of the isolates are resistant to at least one family of antimicrobials, and 11.7% were resistant to all six families. The REP-PCR presented 45 genotypes encoded from R1 to R45, the ERIC-PCR presented 44 genotypes encoded from E1 to E44 and BOXAIR presented 56 genotypes encoded from B1 to B56, dispersed in the departments of Lima, Ica and Libertad (Peru). The study allowed to typify Gallibacterium sp and the combined use of genotypic and phenotypic methods allowed a more precise discrimination between potentially different isolates with moderate genetic diversity in the cities of Lima, Libertad and Ica. 

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Published

2020-11-24

Issue

Vol. 31 No. 4 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Rony Yunior Cotaquispe Nalvarte, Ysabel Koga Yanagui, Robert Tinoco Romero

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Cotaquispe Nalvarte, R. Y., Koga Yanagui, Y., & Tinoco Romero, R. (2020). Genotyping and phenotyping of Gallibacterium sp from poultry with respiratory clinical signs. Revista De Investigaciones Veterinarias Del Perú, 31(4), e19019. https://doi.org/10.15381/rivep.v31i4.19019