Sperm DNA fragmentation index of alpaca (Vicugna pacos) using the sperm chromatin dispersion test

Authors

  • Nancy Huanca Escuela Profesional de Zootecnia, Facultad de Ciencias Agrarias, Universidad Nacional de San Antonio Abad de Cusco
  • César Ordoñez Escuela Profesional de Zootecnia, Facultad de Ciencias Agrarias, Universidad Nacional de San Antonio Abad de Cusco
  • Enrique Ampuero Escuela Profesional de Zootecnia, Facultad de Ciencias Agrarias, Universidad Nacional de San Antonio Abad de Cusco
  • Hernán Cucho Escuela Profesional de Zootecnia, Facultad de Ciencias Agrarias, Universidad Nacional de San Antonio Abad de Cusco

DOI:

https://doi.org/10.15381/rivep.v31i4.19025

Keywords:

alpaca, DNA fragmentation, SCD, semen collection

Abstract

The aim of this study was to analyse the DNA fragmentation in alpaca spermatozoa recovered from the vas deferens deviation (VDD), as well as those collected by electroejaculation (EE) and by post-copula (PC). The sperm was collected from three males per method and three times per animal. Volume, motility, concentration, vitality, functionality of the spermatic membrane and sperm DNA fragmentation index were evaluated. Microscopic variables were evaluated with the Integrated Semen Analysis System - ISAS®; the sperm DNA fragmentation index (DFI) was determined by the chromatin dispersion method (VDD). The DFI data was analysed using a completely randomized block design. An DFI of 36.44 ± 22.50% was found in the VDD method and 13.65 ± 18.98% and 8.42 ± 10.28% for sperm collected by EE and PC, the latter two being superior (p<0.05) to the VDD method. A lower percentage of sperm DNA fragmentation was found in semen collected by post-copulation and electroejaculation.

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Published

2020-11-24

Issue

Vol. 31 No. 4 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Nancy Huanca, César Ordoñez, Enrique Ampuero, Hernán Cucho

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Huanca, N., Ordoñez, C., Ampuero, E., & Cucho, H. (2020). Sperm DNA fragmentation index of alpaca (Vicugna pacos) using the sperm chromatin dispersion test. Revista De Investigaciones Veterinarias Del Perú, 31(4), e19025. https://doi.org/10.15381/rivep.v31i4.19025