Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru

Authors

  • Manuela Vivanco S. Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Adhemir Valera A. Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Luis Llanco A. Grupo SANIVET, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima
  • Enrique Serrano-Martínez Grupo SANIVET, Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima

DOI:

https://doi.org/10.15381/rivep.v31i4.19044

Keywords:

Perkinsus, Argopecten purpuratus, PCR

Abstract

The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.

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Published

2020-12-21

Issue

Vol. 31 No. 4 (2020)

Section

Comunicaciones

License

Copyright (c) 2020 Manuela Vivanco S., Adhemir Valera A., Luis Llanco A., Enrique Serrano-Martínez

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Vivanco S., M., Valera A., A., Llanco A., L., & Serrano-Martínez, E. (2020). Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru. Revista De Investigaciones Veterinarias Del Perú, 31(4), e19044. https://doi.org/10.15381/rivep.v31i4.19044