Basic and functional characterization of the Peruvian Hairless Dog semen

Authors

  • Alexei Santiani Laboratorio de Biotecnologías Reproductivas y Celulares, Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur. Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos
  • María Pérez- Rosales Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Carlos Challco Laboratorio de Biotecnologías Reproductivas y Celulares, Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur, Lima

DOI:

https://doi.org/10.15381/rivep.v31i4.19251

Keywords:

Peruvian hairless dog, semen, spermatozoa, flow cytometry

Abstract

The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values ​​were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm viability; 85.9 ± 5.1% high MMP; 83.6 ± 14.9% acrosomal integrity. These results represent the first report on the seminal characteristics of this breed and could be taken as reference values ​​for a better selection of breeding males and for the diagnosis of reproductive alterations of the breed.

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Published

2020-12-21

Issue

Vol. 31 No. 4 (2020)

Section

Artículos Primarios

License

Copyright (c) 2020 Alexei Santiani, María Pérez- Rosales, Carlos Challco

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Santiani, A., Pérez- Rosales, M., & Challco, C. (2020). Basic and functional characterization of the Peruvian Hairless Dog semen. Revista De Investigaciones Veterinarias Del Perú, 31(4), e19251. https://doi.org/10.15381/rivep.v31i4.19251