Antibiotic resistance in Vibrio spp isolated from shrimp Litopenaeus vannamei. Treatment alternatives with extracts of neem (Azadirachta indica) and oregano (Origanum vulgare)

Authors

  • Luis E. Aguirre Chanta Facultad de Ingeniería Pesquera y Ciencias del Mar, Universidad Nacional de Tumbes
  • Héctor A. Sánchez-Suárez Facultad de Ciencias Agrarias, Universidad Nacional de Tumbes https://orcid.org/0000-0003-2395-5056
  • Alberto Ordinola-Zapata Universidad Nacional de Tumbes https://orcid.org/0000-0002-9644-0531

DOI:

https://doi.org/10.15381/rivep.v32i4.19386

Keywords:

shrimp, antibacterial resistance, phytobiotics, neem, oregano

Abstract

The study aimed to characterize the antibiotic resistance in Vibrio spp isolated from Litopenaeus vannamei and to test oregano (Origanum vulgare) and neem (Azadirachta indica) extracts for their inhibition. Farmed shrimp were collected in Santa Rosa (Ecuador) and samples of hepatopancreas, hemolymph and lesions were taken. Fourteen strains of Vibrio spp were isolated. Another 14 strains of Vibrio spp isolated from farmed shrimp from Tumbes (Peru) were obtained through a donation. The antibiotic resistance of the strains against 10 antibiotics and the sensitivity of four of them (two resistant and two multi-resistant to antibiotics) were evaluated using neem and oregano aqueous extracts (EA), ethanolic (EE), as well as purified with soxhlet and rota evaporator (EPSR). The 28 strains studied were resistant to at least one of the antibiotics, 64.3% were multi-resistant and 50% were resistant to oxytetracycline. Neem and oregano EA and EE did not inhibit any of the four strains, but the undiluted neem EPSR inhibited one of the four strains tested, showing a greater inhibitory effect even than oxytetracycline in it. Undiluted oregano EPSR inhibited all four strains tested. It is concluded that there is high antibiotic resistance in Vibrio spp isolated from farmed shrimp in Santa Rosa and Tumbes; that the EPSR of oregano is an interesting alternative to inhibit Vibrio spp resistant to antibiotics, as well as that the EPSR of neem could be used to inhibit some strains of Vibrio spp resistant to oxytetracycline.

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Author Biography

  • Alberto Ordinola-Zapata, Universidad Nacional de Tumbes

    Ingeniero Pesquero y Maestro en Acuicultura y Gestión Ambiental. Cuenta con estudios culminados de Doctorado en Ciencias Ambientales, en la Maestría en Educación con mención en docencia en el nivel superior. y en la Maestría en Biotenología Molecular. Profesor principal del pregrado y posgrado en la Facultad de Ingeniería Pesquera y Ciencias del Mar de la Universidad Nacional de Tumbes estando a cargo de diferentes asignaturas relacionadas con el área de investigación científica. Ha realizado investigaciones en patología de langostinos, así como diversos estudios en cangrejo del manglar (Ucides occidentalis). Ha participado en investigaciones en el área de biotecnología, conservación ambiental y acuicultura. Tiene inteterés en la investigacion en biotecnología, acuicultura y conservación ambiental. Ocupó el primer puesto en rendimiento académico en sus estudios de pregrado en la Escuela de Ingeniería Pesquera de la Universidad Nacional de Tumbes, así también ocupó el primer puesto en rendimiento académico en la Maestría en Acuicultura y Gestión Ambiental de la Universidad Nacional de Tumbes.

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Published

2021-08-24

Issue

Vol. 32 No. 4 (2021)

Section

Artículos Primarios

License

Copyright (c) 2021 Luis E. Aguirre Chanta, Héctor A. Sánchez-Suárez, Alberto Ordinola-Zapata

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Aguirre Chanta, L. E., Sánchez-Suárez, H. A., & Ordinola-Zapata, A. (2021). Antibiotic resistance in Vibrio spp isolated from shrimp Litopenaeus vannamei. Treatment alternatives with extracts of neem (Azadirachta indica) and oregano (Origanum vulgare). Revista De Investigaciones Veterinarias Del Perú, 32(4), e19386. https://doi.org/10.15381/rivep.v32i4.19386